3-Methyladenine (3-甲基腺嘌呤） 返回列表页

3-Methyladenine  (3-甲基腺嘌呤）

注：本产品仅用于科研，不可用于临床

生物活性

3-Methyladenine (3-MA) is a PI3K inhibitor. 3-Methyladenine is a widely used inhibitor of autophagy via its inhibitory effect on class III PI3K

IC₅₀ & Target^[1]

体外研究
(In Vitro)

3-Methyladenine (0-10 mM; 0-48 hours) induces caspase-dependent cell death in HeLa cells in a time-and dose-dependent manner^[2].
3-Methyladenine (5 mM; 24 hours) suppresses autophagy in HeLa cells under both glucose-free conditions and normal conditions^[2].
3-Methyladenine (5 mM; 0-48 hours) suppresses conversion of LC3-I to LC3-II (autophagy markers) between 12hours and 48 hours, confirms the inhibitory effects on autophagy^[2].
3-Methyladenine induces cell death is independent of autophagy inhibition^[2].
3-Methyladenine significantly shortens the duration of nocodazole-induced-prometaphase arrest^[2].
Note:
在体外细胞培养实验中，3-MA 常用的浓度为 0.5-10 mM，所以优先推荐直接称取本次实验所需粉末量，用培养基全都溶解，0.22 μm 过滤器过滤除菌之后使用。建议溶液现配现用，尽快用完。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究
(In Vivo)

3-Methyladenine (1.5 mg/100 g; intraperitoneal injection; 3-24 hours) treatment alleviates sodium taurocholate-induced severe acute pancreatitis (SAP) in rats at both 12 hours and 24 hours^[3].
3-Methyladenine inhibits autophagy of pancreatic acinar cells in sodium taurocholate-tnduced SAP^[3].
3-Methyladenine also shows inhibitory effects on PI3K/Akt signaling pathway and NF-κB signaling pathway in sodium taurocholate-induced SAP^[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

149.15

性状

Solid

Formula

C₆H₇N₅

CAS 号

5142-23-4

中文名称

3-甲基腺嘌呤

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

溶解性数据