Product Name 

Anti-Engrailed and Invected antibody [4D9]抗体说明书

Product type 

Primary antibodies

Description 

Mouse monoclonal [4D9] to Engrailed and Invected

Immunogen 

C-terminal two-thirds of the invected protein were generated in E. coli with the T7 polymerase expression system.

Reacts with 

Chk, Cyfsh, Dm, Xl, Zfsh

Does not react with 

Ms

Specificity 

This antibody recognizes both the engrailed and invected gene products of Drosophila. The antibody is useful for examining the patterning of segmentation from the cellular blastoderm stage onward. Staining is also seen in a large number of CNS neurons including most of the median neuroblast progeny as well as a small number of PNS neurons.

Epitope 

Epitope has been localized to residues 38-58 of the homeodomain.

Tested applications 

IHC-FoFr

Application notes 
（see key）

Recommended dilutions
IHC-FoFr: 1/200. Fix with 4% paraformaldehyde in PBS for 1 hour.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Specific protocols
Recommended IHC protocol with ab12454

Research areas 

Chromatin and Nuclear Signaling >> Transcription >> Other factors
Neuroscience >> Neurology process >> Neurogenesis

Relevance 

In Drosophila, the Engrailed gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Expression studies have suggested that Engrailed may also function early in organizing the preblastoderm and later in neurogenesis. Engrailed encodes a homeodomain-containing protein that can function as a transcription factor.
Invected is homologous to Engrailed and the gene lies adjacent to that for Engrailed. The function of Invected is unclear.

Alternative names Anti-Engrailed and Invected antibody [4D9] （ab12454）

Database links 

The links below go to external sites and will open in a new browser window

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

4D9

Myeloma 

NS1

Isotype 

IgG1

Light chain type 

kappa

Purity 

IgG fraction

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: PBS, pH 7.4

Material safety datasheet （MSDS） for this product:
Sodium azide MSDS

Form 

Liquid

Concentration 

2.000 mg/ml

Storage instructions 

Store at +4°C short term （1-2 weeks）. Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.