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Anti-BSA/Bovine Serum Albumin抗体*

2013-12-12  阅读(662)

Anti-BSA/Bovine Serum Albumin

From:ABCAM

Catalog Number :RS-0292R

Quantity size :   0.1ml  (dilute with pH 7.4    0.01 M PBS or antibody diluent ) Background: Fraction V bovine serum albumin. Albumin is one of the most extensively utilized proteins in the medical industry. Its availability, inherent stability, and ligand binding ability make albumin an essential component in the production of many therapeutic drugs, vaccines and diagnostic tests. Albumin is a soluble, monomeric protein which comprises about one half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Mutations in this gene on chromosome 4 result in various anomalous proteins. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. The human albumin gene is 16,961 nucleotides long from the putative 'cap' site to the first poly(A) addition site. It is split into 15 exons which are symmetrically placed within the 3 domains that are thought to have arisen by triplication of a single primordial domain. Albumin is synthesized in the liver as preproalbumin which has an N terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin

.Specificity : Anti-BAS is a rabbit polyclonal antibody               

specific for BAS of cow

         use for western blotting,elisa,immunoprecipitation and immunohistochemistry

         Protein G affinity chromatography purification, purity :>95%

         Isotype:  IgG

         mol wt:  69kDa

Application :  

         WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 lmmunohistochem1100-500

         BSA

        not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user..

Storage:  Store at -20 for one year. Avoid repeated freeze/thaw cycles. The  lyophilized antibody is stable at room temperature for at least one month and for greater  than a year when kept at -20. When reconstituted in sterile pH 7.4 0.01M PBS or diluent  of antibody, the antibody is stable for at least six weeks at 2-4

 

Important Note: This product as supplied is intended for research use only, not for use in  human, therapeutic or diagnostic applications.



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