英文名称：Hotstart Taq DNA Polymerase
其他名称：Hotstart Taq DNA聚合酶
分子量：94kDa，单体
级别：BR
纯度：≥99%
浓度：5u/ul 
活性定义：在74℃、30分钟内，使10nmol脱氧核糖核酸合成渗入多聚核苷酸（吸附在DE-81上）所需的酶量
酶活性分析混合物：25mM TAPS(PH9.3,25℃), 50mM KC1, 2mM MgCL2, 0.2mM各种dATP,dGTP,dTTP, 0.1mM dCTP, 0.75mM活化的鲑鱼精DNA和0.4MBq/ML(3H).-dTTP
保存缓冲液组分：20mM Tris-HCL(PH9.0), 1mM DTT, 0.1mM EDTA,100mM KC1, 0.5%(V/V) Tween 20，0.5%(v/v) Nonidet P40和50%(v/v)甘油
10×Hot start PCR Buffer：200mM Tris-HCL(PH8.3,25℃), 200mM KC1, 50mM (NH4)2SO4
抑制剂：阴离子去污剂（脱氧胆酸、肌氨酰和SDS的浓度分别高于0.06、0.02和0.01%时）
失活：酚/氯仿抽提
质量控制：相关测试表明无内切或外切脱氧核糖核酸酶、核糖核酸酶污染
功能启动：热启动PCR
性状(以下信息仅供参考)：液体。设计用于增强DNA扩增的特异性、敏感性和产量。使用该酶可在室温配制反应体系，使用方便。它是一种化学修饰的重组Taq DNA Polymerase，蛋白分子氨基酸残基上增加了热不稳定的封闭基团。该酶在室温下没有活性，避免形成引物二聚体和非特异性延伸，从而增加DNA扩增的特异性。95℃加热4分钟可恢复活性。活化的酶具有与Taq DNA Polymerase相同的功能：催化5——3方向合成DNA，无可检测的3——5校正外切酶活性，具有较低的5——3外切酶活性。该酶还具有脱氧核糖核酸酶转移活性，在PCR产物的3-端多加11个腺嘌呤，活化前检测不到这些活性。
用途：本品仅供科研，不得用于其它用途。(以下用途仅供参考)高产量扩增复杂模板；PCR特异性高-降低错配效应和减少引物二聚体的生成；增强的PCR敏感性；使用方便，室温配制PCR反应体系；扩增产物具有3-dA突出末端；热启动PCR；高产量扩增长达3kb片段；RT-PCR；特异性扩增复杂cDNA和基因组模板；扩增低拷贝DNA模板；实时PCR；多重PCR；制备TA克隆用PCR产物
保存：-20℃，保质期1年
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