FAM tetrazine,FITC-tetrazine,荧光素染料

Tetrazine (methyltetrazine) derivative of fluorescein, pure 5-isomer.

Tetrazines are reactive heterodiene ponents for [4+2] inverse electron demand Diels-Alder reaction (ied-DA) with strained cycloalkenes (trans-cyclooctenes, cyclopropenes). This reaction is also called TCO (tetrazine-cyclooctene) ligation. The reaction is among the fastest bioconjugation reactions.

General properties

Appearance:	yellow solid
Mass spec M+ increment:	529.12
Molecular weight:	559.53
Molecular formula:	C31H21N5O6
Solubility:	good in DMF, DMSO
uality conrol:	NMR 1H, HPLC-MS (95%)
Storage conditions:	Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

Spectral properties

Excitation maximum, nm:	490
ε, L⋅mol−1⋅cm−1:	80000
Emission maximum, nm:	513
Fluorescence quantum yield:	0.93
CF260:	0.20
CF280:	0.17

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伯胺 Primary amine

BDP FL amine

Cyanine3 amine

Cyanine5 amine

Cyanine5.5 amine

Cyanine7 amine

Cyanine7.5 amine

Sulfo-Cyanine5 amine

Sulfo-Cyanine7 amine