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西安凯新生物科技有限公司>荧光染料及其标记物>TAMRA羧基四甲基罗丹明>5-TAMRA-N35-TAMRA Picolyl Azide,Alexa Fluor 546
5-TAMRA Picolyl Azide,Alexa Fluor 546
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  • 5-TAMRA Picolyl Azide,Alexa Fluor 546

产品参考价:¥2050 举报
产品型号: 5-TAMRA-N3
厂商性质: 生产商
地: 西安市
更新时间: 2018-02-11 18:58:39
浏览次数: 233
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产品简介

5-TAMRA Picolyl Azide,Alexa Fluor 546
目前Cyanine系列染料以两种异构体的形式存在,一种是非磺化花青素(non-sulfonated cyanines),一种是磺化花青素(sulfonatedcyanine)。两者的光谱性质几乎一致,因此大部分应用可相互替换。

详细介绍

5-TAMRA Picolyl Azide,Alexa Fluor 546

产品介绍

5-TAMRA Picolyl Azide

5-TAMRA Picolyl Azide is an advanced fluorescent probe that incorporates a copper-chelating motif to raise the effective concen*ration of Cu(I) at the reaction site. The chelating effect of picolyl azide moiety to raise the effective concen*ration of copper at the reaction site is so great that it leads to an increase of signal intensity of up to 40-fold. In addition, the use of 5-TAMRA Picolyl Azide instead of conventional 5-TAMRA Azide allows for at least a tenfold reduction in the concen*ration of the copper catalyst without sacrificing the efficiency of labeling.

Although the mixed isomers of 5(6)-TAMRA is a preferred, routinely used red fluorescent dye for staining proteins, labeling peptides and nucleotides with TAMRA mixed isomers might be troublesome due to significant signal broadening in HPLC purification. Peptides and nucleotides labeled with a single isomer TAMRA usually give better resolution in HPLC purification that is often required in the conjugation processes.

In summary, the in*roduction of a picolyl moiety into an azide probe leads to a substantial increase in the sensitivity of alkyne detection. This will be of special value for the detection of low abundance targets or where significant increase in signal intensity is desired.

Abs/Em Maxima

545/576

Extinction coefficient

91,000

Flow cytometry laser line

532 or 555 nm

Microscopy laser line

532 or 555 nm

Spectrally similar dyes

Alexa Fluor® 546, Atto™ 543, CF™ 555 Dye, DyLight™549

Molecular weight

808.89

CAS

N/A

Solubility

DMSO, DMF, MeOH

Purity

>97% (HPLC)

Appearance

Red solid

Storage conditions

-20C

Shipping conditions

Ambient temperature

结构式:

 

5-TAMRA Picolyl Azide,Alexa Fluor 546

西安凯新生物科技有限公司

联系电话:029-68881181
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炔烃 Dye alkynes

1-Ethynyl pyrene

3-Ethynyl perylene

BDP FL alkyne

FAM alkyne, 5-isomer

FAM alkyne, 6-isomer

TAMRA alkyne, 5-isomer

BDP TMR alkyne

Cyanine3 alkyne

Cyanine5 alkyne

Cyanine5.5 alkyne

Cyanine7 alkyne

Cyanine7.5 alkyne

Sulfo-Cyanine3 alkyne

Sulfo-Cyanine5 alkyne

5-TAMRA Picolyl Azide,Alexa Fluor 546

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