5-EU,5-Ethynyl Uridine,尿嘧啶炔烃标记物
5-Ethynyl Uridine (5-EU)
5-Ethynyl uridine (5-EU) is a metabolic labeling reagent used to detect global RNA transcription temporally and spatially in both in-vitro and in-vivo with the modified nucleotide using two-step techniue.
In step one, the alkyne-containing uridine analog 5-EU is fed to cells or animals and actively incorporated into RNA. In step two the incorporated 5-EU is detected via chemoselective ligation or &lduo;click&rduo; reaction between an azide and an alkyne where the modified biomolecules are detected with a corresponding azide-containing dye or hapten. Cells can be analyzed by fluorescence microscopy, flow cytometry or high-content imaging and analysis (HCS).
Molecular weight | 268.22 |
Chemical position | C11H12N2O6 |
CAS | 69075-42-9 |
Solubility | Water, DMSO, DMF |
Purity | >95% (HPLC) |
Appearance | Off white to grey crystalline |
Storage conditions | -20C |
Shipping conditions | Ambient temperature |
5-EU,5-Ethynyl Uridine,尿嘧啶炔烃标记物
公司：西安凯新生物科技有限公司
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