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详细介绍
徕卡倒置显微镜DMIL LED产品介绍：
LEICA DM IL LED – BRILLIANT OPTICS COMBINED WITH INNOVATIVE ILLUMINATION*The Leica DM IL LED is exceptionally well-suited for uses ranging from various cell and tissue culture examinations in life sciences, developmental biology studies or micromanipulation in cell biology to living cell examinations in transgenics or electro-physiology.
* The fluorescence version, the Leica DM IL LED Fluo, also offers a variety of possible applications. Optionally, it is also available with the new LED illumination.
* Heatable microscope stages and 3-plate cross-stages provide great fl exibility for experiments on living cells under physiological conditions.
* The Leica DM IL LED has a further advantage that distinguishes it from other microscopes in its class: The stａnd is highly com-patible with components of the Leica research microscopes. Objectives, eyepieces, tubes, camera ports, contrast methods. Additionally, special tubes and condensers have been developed for the Leica DM IL LED.
Integrated fl uorescence
* Manual fl uorescence with three fi lter cubes.
* Integrated shutter.
* Optionally LED, classic mercury illumination or fiber optic coupling
Flexible and modular
* A full range of optical components
* Compatible with research stands
* Unheated and heated stages
* Large selection of tubes
* Comprehensive range of accessories for special applications
The Most Comprehensive Array of Contrast Methods
All available contrast methods can be adapted to individual applications easily and quickly. Two condensers have been devel-oped specifi cally for the Leica DM IL LED, which can be used for the entire magnifi cation range of the respective contrast method. The high-resolution S40/0.45 condenser makes even tiny details of a specimen visible. Both condensers, the S40/0.45 and S80/0.30, allow for use of phase contrast up to the 63x objective as well as Integrated Modulation Contrast (IMC) up to the 40x objective.
Fatigue-free operation
The ergonomic arrangement of all controls such as the focus dial, brightness controller, condenser height adjustment, objective nosepiece and XY stage adjustment allow users to be relaxed while working with the microscope – even for hours. The height-adjustable stages, Ergo tubes with variable tube height, fl exible viewing height, and the interpupillary distance and diopter setting enable each user to confi gure his or her personal Leica DM IL LED. The large working distances provide suffi cient room for large cul-ture fl asks, and the unobstructed view of the specimen area fa-cilitates handling more diffi cult specimens.
Bright field
All Leica brightfi eld and phase objectives from 2.5x to 100x can be used for stained specimens. The bright fi eld method can be used even for low magnifi cation levels without condenser. A working distance of 200 mm is realized simply by unscrewing the condenser head.
Phase contrast
Phase contrast is used primarily in live cell microscopy to make structures in unstained specimens visible. Three preadjusted light rings on a slider allow phase contrast for all objectives from 5x to 63x. No readjustment is necessary when changing the objectives. The intelligent LED illumination adjusts the brightness automati-cally when switching between the phase contrast and brightfi eld method.
Integrated Modulation Contrast (IMC)
IMC creates relief-type images and has proven to be an alter-native to Differential Interference Contrast (DIC), particularly in micromanipulation. The IMC developed by Leica Microsystems does not require special objectives because the IMC modulator is not integrated into the objective, but operated via a separate slider. The IMC illumination slider is encoded and controls the LED illumi-nation. IMC is available for both condensers and for standard 10x, 20x, 32x and 40x objectives.
Fluorescence
Incident-light fl uorescence is an integral part of the Leica DM IL LED Fluo microscope variant. The fl uorescence slider holds three fi lter blocks. The transmitted light method and fl uorescence can be used simultaneously. This way, object structures can be clearly assigned. Each fi lter block comprises an optimally matched combi-nation of excitation, refl ection and barrier fi lters. Illumination can be generated via the Leica SFL100 LED illumination, the classic mercury illumination or the Leica EL6000 fi ber optic coupling.
Longworking Distance Condenser & Perfectly Illuminated
For the fi rst time, a condenser concept has been realized in the Leica DM IL LED that allows all contrast methods with all con-densers. With a working distance of at least 40 mm and a numeri-cal aperture of 0.45, the S40 condenser is the perfect tool for applications for which optimum resolution is the most important parameter. Phase contrast and IMC ensure optimum contrasting.
The working distance of at least 80 mm and 0.30 aperture of the S80 condenser are the ideal prerequisites for achieving maximum possible free room around the specimen and optimum contrasting at the same time. The continuous adjustment of the condenser height depending on specimen vessel and liquid layer is a one-of-a-kind feature. It ensures maximum fl exibility when using periph-eral microtools.
Whether you work with thin sections or thick specimens, phase and modulation contrast produce a brilliant microscopic image for all specimens and applications.
Flexible Fluorescence
Fluorescence applications, in particular GFP labeling, play an ever more important role in clinical diagnostics and routine microscopy. The Leica DM IL LED Fluo has been designed in consideration of this trend. The microscope has been equipped with a fl uorescence axis and a 3-position slider to ensure fast and easy switching to different fl uorochromes. The slider glides smoothly in an elabo-rately constructed dovetail guide. An extensive, constantly grow-ing range of fi lters allows a wide variety of fl uorescence exami-nations. The fi lter blocks are optimized for minimizing stray light. Excitation, refl ection and barrier fi lters adapt to your application. Transmitted-light methods can be used simultaneously or as an alternative so that fl uorescent and nonfl uorescent structures can be clearly assigned. An integrated shutter protects the specimen against bleaching.
The Leica DM IL LED Fluo is the fi rst routine fl uorescence microscope that allows users to choose between classic illumination (halogen, mercury or high-pressure xenon lamps), the ”cold” light guide cou-pling of the Leica EL6000 and the new LED illumination, Leica SFL100. This gives users the ability to excite and examine fl uorochromes to see more detail under the microscope. A dark background and the bright emitted fl uorescence produce brilliant color images. The fi eld of applications ranges from DAPI (UV) for nuclear staining to CY5 (IR) for immuno-histochemical arrays. The Leica DM IL LED Fluo thus is a high-performance instrument for use in immunology, cytology, virolo-gy – and in any field that requires fluorescence techniques for living specimens.
Apart from the Ergo Modules for variable height adjustment, Leica Microsystems offers a drawing attachment for special examina-tions and a discussion attachment for two observers.
In live cell microscopy, the right microscope stage and the cor-responding accessories are important prerequisites for the best results. In addition to the fi xed stages with or without mechanical stage, Leica Microsystems offers 3-plate cross-stages with differ-ent inserts for a wide variety of culture fl asks. All microscopes are also available with heating stages or heating inserts. The sturdy mechanical system and the compact stａnd ensure best possible stability.
徕卡倒置显微镜DMIL LED基本参数：
| Leica DM IL LED | |
|---|---|
| Optics | Infi nity corrected (HCS), tube factor 1x |
| Field of view | 20 mm |
| Lamp power supply | AC input: 100-240 V /0.33-0.19 A DC output: 5 V /2 A |
| Illumination | 5 watt LED |
| Focusing | Coarse and fi ne adjustment, nosepiece focusing, vertical travel 7 mm |
| Objective Nosepiece | 4-position, M25x0.75 objective thread |
| Stage | Fixed work stage with 3-point support 248 x 212 x 20 mm |
| Heating stage 248 x 212 x 20 mm incl. TempControl 2000 | |
| 3-plate stage, 150 x 150 mm insert plate, adjustment range 60 x 40 mm | |
| Transmitted-light illuminator arm | with illumination unit, with precentered LED illumination incl. collector, diffusion filter, iris aperture diaphragm, condenser holder |
| Additionally for fl uorescence versions: | Lamp housing: Interchangeable lamp housings for fl uorescence |
| Fluorescence: Integrated lamp mount in a massive, stable back panel, integrated fl uorescence axis, 3-position fl uorescence slide for three different fi lter blocks, dark stop | |
Overview of the Leica DM IL LED
| Optics | Leica HC optics (infi nity corrected) HC objectives: 2.5x–100x | DM IL LED Fluo | DM IL LED | |
| Objective Nosepiece | Four positions | ● | ● | |
| Focus | Coaxial coarse and fi ne adjustment, travel path 7 mm, nosepiece focusing | ● | ● | |
| Transmitted-light illuminator | 5 watt LED, power supply (in 100-240, out 5 V/2 A) Filter holder for TL fi lter Ø 32 mm, collector, scattering fi lter | ● | ● | |
| Condenser | Interchangeable condenser heads: S40/0.45: available working distance 40 mm, aperture 0.45 S80/0.30: available working distance 80 mm, aperture 0.30 | ● | ● | |
| Contrasting | Precentered insert with four positions Insert for IMC illumination | (Brightfi eld, 5x–63 x Phase Contrast)(Brightfi eld, 10x, 20x, 32x, 60x IMC) | ● | ● |
| Contrast methods | Brightfi eld, Phase Contrast, Integrated Modulation Contrast | ● | ● | |
| Fluorescence | Fluorescence slider with three positions for fi lter cubes Manual light stop | ● | ○ | |
| Fluorescence illumination | Fluorescence LED Leica SFL100, 50W Hg, 100W Hg, Leica EL6000 fiberoptic coupling | ● | ○ | |
| Stages | Fixed stage, fixed heating stage, 3-plate stage, attachable mechanical stage for both fixed stages | ● | ● | |
| Documentation | Camera port for all Leica digital cameras and common camera models | ● | ● | |
| Tubes | Binocular tube 45°, interpupillary distance 55–75 mm, fi eld of view 20 mm | ● | ● | |
| Tubes | Trinocular phototube 45°, interpupillary distance 55–75 mm, fi eld of view 20 mm, | ● | ● | |
| with camera port positioned 88 mm to the side, selectable photo or visual | ● | ● | ||
Additional options in the Leica DM product line: Standard binocular tube 30°, Ergo binocular tube 15° Ergo Vario binocular tube 7.5–15°, Ergo Vario binocular tube 0–55° Ergo Vario binocular tube 5°–32° and eyepiece extension 0–30 mm Standard trinocular phototube 30°, Ergo Vario trinocular phototube 0°–35°
| ● | ● | ||
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