当前位置：杭州昊鑫生物科技股份有限公司>>云克隆>> SEA861Hu补体成分3(C3)检测试剂盒
| 产地 | 进口 | 规格 | 48T |
|---|---|---|---|
| 级别 | 化工级 | 证书 | ISO系列证书 |
编号SEA861Hu
物种Homo sapiens (Human，人) 相同的名称，不同的物种。
实验方法双抗夹心
反应时长3h
检测范围7.8-500ng/mL
灵敏度z小可检测剂量小于等于3.7ng/mL.
样本类型serum, plasma and other biological fluids
下载英文说明书 中文说明书
规格48T96T 96T*5 96T*10 96T*100
本试剂盒用于检测补体成分3(C3)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
分别于定值血清及血浆样本中加入一定量的补体成分3(C3)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 78-92 | 88 |
| EDTA plasma(n=5) | 87-102 | 92 |
| heparin plasma(n=5) | 93-101 | 97 |
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
在定值血清及血浆样本内加入适量的补体成分3(C3)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中补体成分3(C3)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 90-98% | 81-98% | 91-98% | 95-105% |
| EDTA plasma(n=5) | 91-105% | 89-98% | 99-105% | 88-102% |
| heparin plasma(n=5) | 79-93% | 89-96% | 89-96% | 78-92% |
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。
将补体成分3(C3)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的补体成分3(C3)与连接于固相载体上的抗体结合，然后加入生物su化的补体成分3(C3)抗体，将未结合的生物su化抗体洗净后，加入HRP标记的亲和素，再次洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成Z终的黄色。颜色的深浅和样品中的补体成分3(C3)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。
| 杂志 | 参考文献 |
| Artificial Organs | Experimental Research With Synthetic Copolymer-Coated Cardiopulmonary Bypass Circuits: Inflammatory and Thrombogenicity Analysis [Wiley: source] |
| Journal of Clinical Immunology | Complement activation contributes to the injury and outcome of kidney in human anti-glomerular basement membrane disease. [PubMed: 22941511] |
| Lipids in Health and Disease | Beneficial effects of omega-3 fatty acids in the proteome of high-density lipoprotein proteome [PubMed: PMC3480889] |
| Am J Reprod Immunol | Human IgG Antinuclear Antibodies Induce Pregnancy Loss in Mice by Increasing Immune Complex Deposition in Placental Tissue: In Vivo Study [PubMed: 26388133] |
| Veterinary Immunology and Immunopathology | Oral administration of heat-inactivated Mycobacterium bovis reduces the response of farmed red deer to avian and bovine tuberculin [Pubmed:27032499] |
| International Journal of Molecular Sciences | Identification of Serum Peptidome Signatures of Non-Small Cell Lung Cancer [Pubmed:27043541] |
| Food Chem. | Comparative proteomics of milk fat globule membrane in goat colostrum and mature milk [Pubmed:27173528] |
| Scientific Reports | Generation of complement protein C3 deficient pigs by CRISPR/Cas9-mediated gene targeting. [pubmed:28694465] |
| Veterinary Microbiology | The response of red deer to oral administration of heat-inactivated Mycobacterium bovis and challenge with a field strain. [pubmed:28888638] |
| Proteomics Clinical Applications | iTRAQ‐Based Proteomic Analysis Reveals Protein Profile in Plasma from Children with Autism [Pubmed:29274201] |
| Nanomaterials | An OMV-Based Nanovaccine Confers Safety and Protection against Pathogenic Escherichia coli via Both Humoral and Predominantly Th1 Immune Responses in … [Pubmed: 33233490] |
| Diagnostics | Systemic Alterations of Immune Response-Related Proteins during Glaucoma Development in the Murine Model DBA/2J [Pubmed: 32585848] |
| Scientific Reports | The bovine cumulus proteome is influenced by maturation condition and maturational competence of the oocyte [Pubmed: 32555221] |
| Int Immunopharmacol | Complement component 3 prevents imiquimod-induced psoriatic skin inflammation by inhibiting apoptosis in mice [Pubmed: 32535539] |
| Italian Journal of Animal Science | Effects of chitooligosaccharide supplementation on laying performance, egg quality, blood biochemistry, antioxidant capacity and immunity of laying hens during the … [] |
| Dev Comp Immunol | A deep-sea pathogenic Bacillus subtilis isolate employs different strategies to escape the killing of teleost and murine complements [33545212] |
| Animal | Effect of alfalfa hay and starter feed supplementation on caecal microbiota and fermentation, growth, and health of yak calves [33610512] |
| British Journal of Pharmacology | The suppression of Brd4 inhibits peripheral plasma cell differentiation and exhibits therapeutic potential for systemic lupus erythematosus [] |
| Scientific Reports | Maternal Serum Proteomic Profiles of Pregnant Women With Type 1 Diabetes [34940989] |
请输入账号
请输入密码
请输验证码
以上信息由企业自行提供，信息内容的真实性、准确性和合法性由相关企业负责，制药网对此不承担任何保证责任。
温馨提示：为规避购买风险，建议您在购买产品前务必确认供应商资质及产品质量。
制药网