Anti-Brdu

Catalog Number : Rs-0489R

Quantity size :   0.1ml  （dilute with pH 7.4    0.01 M PBS or antibody diluent ）

The immunocytochemical detection of bromodeoxyuridine （BrdU） incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis.

BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis

pecificity :   

- Anti-Brdu is a rabbit polyclonal antibody unconjugated                

specific fo Brdu of  Human, Mouse, Rat, Dog, Horse

–  use for western blotting,elisa,immunoprecipitation and immunohistochemistry

–  Protein G affinity chromatography purification, purity :>95%

–  Isotype:  IgG

–  mol wt:  48 kDa

Application :  

–  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500

–  not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user..

Storage:  Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The  lyophilized antibody is stable at room temperature for at least one month and for greater  than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent  of antibody, the antibody is stable for at least six weeks at 2-4 ℃

Important Note: This product as supplied is intended for research use only, not for use in  human, therapeutic or diagnostic applications.