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分类名称 : 氧化应激测定,代谢产物分析 产品介绍 :

测定方法  比色法（585nm）定量测定过氧化物的浓度。

产品说明  过氧化物（如过氧化氢）是在氧化应激条件下形成的一种重要的活性氧。过氧化物含量高可能与衰老、哮喘、糖尿病、动脉浆样硬化、白内障、关节炎和神经组织退化等疾病有关。在科研和药物研发中，方便、直接、自动化的过氧化物含量检测手段是zui为理想的。本公司研制的过氧化物检测试剂盒可直接用于生物样品的检测，免除了预处理过程。检测原理是利用试剂与过氧化物氧化反应生成紫色复合物的特性，在波长540-610nm (585nm) 下检测，其颜色深浅程度与样品中过氧化物的含量成正比。

产品特点  敏感、准确：用山梨醇加深颜色，在96孔板中测试，双氧水的检测范围在0.4-100 μM（14-3400 ng/mL）。 简单、高通量：整个测试过程只需加入一种工作试剂并反应30分钟。利用96孔板的高通量检测手段，每天可以测试上千份样本。

产品用途  1．直接检测生物样本中过氧化物含量（如，血清、血浆、尿液、细胞溶液和培养液。 2．适用于药理学研究，检测药物对过氧化物代谢的影响。

储藏条件  可在室温下运输。试剂在4℃环境下保存.

DESCRIPTION  Peroxide (e.g. hydrogen peroxide H2O2) is one of the key reactive oxygen species formed under oxidative stress conditions. High levels of peroxide formation have been linked to pathological conditions such as ageing, asthma, diabetes, atherosclerosis, cataract, inflammatory arthritis and neurodegenerative diseases. Simple, direct and automation-ready procedures for quantitative determination of peroxide find wide applications in research and drug discovery. Our peroxide assay kit is designed to measure peroxide concentration in biological samples without any pretreatment. The improved method utilizes a chromogenic reagent, which forms a purple complex with peroxides present in the sample. The intensity of the color, measured at 540-610nm, is an accurate measure of the peroxide level in the sample.

KEY FEATURES  Sensitive and accurate. Enhanced color intensity using sorbitol. Detection range 0.4 μM (14 ng/mL) to 100 μM (3,400 ng/mL) H2O2 in 96-well plate assay. Simple and high-throughput. The procedure involves addition of a single detection reagent and incubation for 30 min. Can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day.

APPLICATIONS  Direct Assays: H2O2 in biological samples (e.g. serum, citrate-plasma, urine, cell lysate, culture medium). Pharmacology: effects of drugs on peroxide metabolism.

STORAGE CONDITIONS  The kit is shipped at room temperature. Store all reagents at 4 °C. Shelf life of at least 6 months.

编号：DIOX-048，DIOX-250

测试：48T,250T