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分类名称 : 血液尿液化学,代谢产物分析 产品介绍 :

测定方法   比色法（565nm）定量测定L -乳酸的浓度。

产品说明   乳酸是在缺氧或厌氧条件下由乳酸脱氢酶(LDH)催化生成。因此乳酸浓度的监测是组织内氧需求与利用平衡的良好指标。同时对细胞和动物生理学研究也很重要。简单，直接和自动化的乳酸浓度检测方法是非常可取的。博世生物技术提供的L-乳酸测试盒是基于乳酸脱氢酶催化氧化乳酸，其中形成的NADH耦合到甲臜(MTT)发色体上。产物的颜色强度(565nm)与样品中乳酸的浓度成正比。

适用范围   直接检测：样品如血清、血浆、细胞培养物中的乳酸浓度。

产品特点    敏感、准确: 96-孔板检测范围为0.05～2 mM乳酸，对于含有酚红的细胞培养物样品，检测范围为0.1 mM～1 mM 乳酸。方便: 仅需添加单一工作试剂并在开始和20分钟时读取光密度。室温下检测，无须37℃加热器。高通量: 通过96-孔板高通量的自动化检测，每天可以检测上千份样品。 

贮藏条件：所有试剂均于-20°C保存。

Description   Lactate is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate levels is, therefore, a good indicator of the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology. Simple, direct and automation-ready procedures for measuring lactate concentration are very desirable. our EnzyChrom^TM lactate assay kit is based on lactate dehydrogenase catalyzed oxidation of lactate, in which the formed NADH reduces a formazan (MTT) reagent. The intensity of the product color, measured at 565 nm, is proportionate to the lactate concentration in the sample.

Applications    Direct Assays: lactate in serum, plasma, and cell media samples.

Key features    Sensitive and accurate. Detection limit of 0.05 mM and linearity up to 2 mM L-Lactate in 96-well plate assay. For cell culture samples containing phenol red: detection limit of 0.1 mM and linearity up to 1 mM L-Lactate in 96-well plate assay.Convenient. The procedure involves adding a single working reagent, and reading the optical density at time zero and at 20 min. Room temperature assay. No 37°C heater is needed.High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.

Storage conditions. Store all reagents at -20°C. Shelf life of at least 6 months (see expiry dates on labels).

其他 :

编号：ECLC-048,ECLC-100

测试：48T,100T