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分类名称 : 血液尿液化学,代谢产物分析 产品介绍 :

测定方法    比色法（340nm）定量测定酮体（乙酰乙酸和3 -羟基丁酸酯）的浓度。

产品说明    酮体（乙酰乙酸和3-羟基丁酸）主要由脂肪酸氧化, 在肝脏内产生，正常情况下尿液和血液中的酮体浓度很低。血液中酮体浓度升高可导致代谢性酸中毒，这往往与糖尿病、儿童低血糖、生长激素缺乏、酒精或水杨酸中毒及先天性代谢缺陷有关。简单、直接、自动化的乙酰乙酸（AcAc）和3-羟基丁酸（BOH）检测手段广受亲睐。本公司生产的酮体测试盒是基于3-羟基丁酸脱氢酶催化反应，在340nm处读取的NADH吸光度的变化便与AcAc 和 BOH的浓度直接相关。

适用范围    直接检测血清、血浆、尿液和其它生物样品中的酮体浓度。

产品特点    敏感且准确：用10mL样品。采用96孔板检测，线性检测范围0.12 - 8 mM酮体。简便：检测过程中只需加入一种工作试剂，并在室温下读取吸光度。高通量：该高通量96孔板检测方法易于自动化，每天可处理大量样品。

储存条件：所有试剂均在-20°C下保存。

Description       Ketone bodies (acetoacetic acid and 3-hydroxybutyric acid) are produced in the liver mainly from oxidation of fatty acids, and are normally present at low concentrations in urine and blood. Increased ketone concentrations in the blood may lead to metabolic acidosis, which has been associated with diabetes, childhood hypo-glycaemia, growth hormone deficiency, alcohol or salicylate intoxication and inborn errors of metabolism.Simple, direct and automation-ready procedures for measuring acetoacetic acid (AcAc) and 3-hydroxybutyric acid (BOH) are very desirable. our EnzyChrom^TM ketone body assay is based on 3-hydroxybutyrate dehydrogenase catalyzed reactions, in which the change in NADH absorbance, measured at 340nm, is directly related to the AcAc and BOH concentrations,

Applications       Direct assays of ketone body in serum, plasma, urine and other biological samples.

Key features        Sensitive and accurate. Uses 10 mL sample. Linear detection range of 0.12 to 8 mM for each ketone body in 96-well plate assay.Convenient. The procedure involves adding a single working reagent, and reading the optical density at room temperature.High-throughput. Can be automated as a high-throughput 96-well plate assay for many samples per day.

其他 :

编号：EKBD-048,EKBD-100