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分类名称 : 代谢产物分析 产品介绍 :

测定方法     比色法（570nm）定量测定脂肪水解的程度。

产品说明  肥胖症是一种慢性疾病，其起因是过剩的能量以脂肪的形式被贮存起来，而过度肥胖容易引发Ⅱ型糖尿病，心血管疾病以及癌症等疾病。脂肪水解是指通过分解甘油三酸酯，使其转变为甘油和游离脂肪酸的过程。在细胞环境下快速、准确地量化脂解作用，在科研和药物研究中应用十分广泛。本公司所生产的脂肪水解检测试剂盒通过加入一种工作试剂，可直接对脂解过程中释放的甘油量进行测量，在570nm条件下，产物的颜色强度与样本中甘油的浓度成正比。

产品特点      敏感、准确：采用96孔板检测，甘油的线性检测范围为：（1）比色法，0.9-100 μg/mL (10-1000 μM)；（2）荧光法，0.2-5 μg/mL。 简便、快速：整个检测过程只需加入一种工作试剂，并在室温下反应20分钟。 高通量：在570nm下进行药物检测，*的降低了潜在干扰，并能兼容含有酚红的培养基。检测可采用96孔板或384孔板。

产品用途  1. 能够直接检测细胞培养基内的甘油含量，从而分析脂肪水解作用。2. 在药物和药理研究中，检测药品对脂肪水解的影响。

储藏条件   -20°C保存，冰运。

DESCRIPTION  Obesity is a chronic condition that develops from storage of excessive energy in the form of adipose tissue. The resulting adiposity presents a high risk factor for diseases such as type 2 diabetes, cardiovascular diseases, and cancer. ADIPOLYSIS or lipolysis is a highly regulated process in fat metabolism, in which triglycerides are broken down into glycerol and free fatty acids. Rapid, robust and accurate procedures for adipolysis quantification in cell culture are very useful in research and drug discovery. Our adipolysis assay kit directly measures glycerol released during adipolysis. This homogeneous assay uses a single Working Reagent that combines glycerol kinase, glycerol phosphate oxidase and color reactions in one step. The color intensity of the reaction product at 570nm is directly proportional to glycerol concentration in the sample.

KEY FEATURES  Sensitive and accurate. Use as little as 10 μL samples. Linear detection range in 96-well plate: 0.9-100 μg/mL (10 to 1000 μM) glycerol for colorimetric assays and 0.2 to 5 μg/mL for fluorimetric assays. Rapid and convenient. The procedure involves addition of a single working reagent and incubation for 20 min at room temperature. Robust and amenable to HTS assays. Potential interference by testing drugs is greatly reduced at 570nm. Compatible with culture media containing phenol red. Assays can be performed in 96 or 384-well plates.

APPLICATIONS  Direct Assays: adipolysis (glycerol in cell culture media). Drug Discovery/Pharmacology: effects of testing drugs on adipolysis.

STORAGE CONDITIONS  The kit is shipped on ice. Store Assay Buffer at 4°C and other reagents at -20°C. Shelf life of two months after receipt.

其他 :

编号：EAPL-048, EAPL-100

测试：48T, 100T