齐一生物供应小鼠降钙素（CT）ELISA试剂盒齐一生物科技有限公司主要经营进口原装Elisa试剂盒，种类齐全，价格实惠，货期短。购买我公司ELISA产品客户，可享免费代测、全程技术指导：(包括售前标本收集、后期实验、实验结束后数据分析等技术问题，我公司会有专业的技术即时给您去电沟通、解决。)保证售后服务。

齐一生物供应小鼠降钙素（CT）ELISA试剂盒操作步骤:
1.包被过程(注意设置空白对照,阴性对照):
将所用抗原用包被稀释液稀释到适当浓度(一般所需抗原包被量为每孔20-200μg),每孔抗原加入100μl,置37℃,4h,或4℃,24h;弃去孔中液体.(为避免蒸发,板上应加盖或将板平放在底部有湿纱布的金属湿盒中)
2.封闭酶标反应孔:
5%小牛血清置37℃封闭40min.封闭时将封闭液加满各反应孔,并去除各孔中的气泡,封闭结束后用洗涤液满孔洗涤3遍,每遍3min.
洗涤方法:吸干孔内反应液,将洗涤液注满板孔,放置2min略作摇动,吸干孔内液,倾去液体后在吸水纸上拍干
洗涤次数3次
3.加入待检测样品(建立合适的浓度梯度):
检测时一般采用1:50-1:400的稀释度,应采用较大稀释体积进行,一般保证样品吸取量>20μl.
将稀释好的样品加入酶标反应孔中,每样品少加双孔,每孔100μl,置于37℃,40-60min.用洗涤液满孔洗涤3遍,每遍3min.
4.加入酶标抗体:
酶标抗体:根据酶结合物提供商提供的参考工作稀释度进行.37℃,30-60min之间.短于30min往往结果不稳定.每孔加100μl.洗涤同前.
5.加入底物液(现用现配):
*TMB-过氧化氢尿素溶液,OPD-过氧化氢底物液系统次之.
底物加入量:每孔100μl,置37℃避光放置3-5分钟,加入终止液显色.
6.终止反应:
每孔加入终止液50μl终止反应,于20min内测定实验结果.
7.结果判断:OPD显色后采用492nm波长,TMB反应产物检测需要450nm波长.检测时一定要首先进行空白孔系统调零.用测定标本孔的吸收值与一组阴性标本测定孔平均值的比值(P/N)表示,当P/N大于2时作为抗体的效价.(数值的大小依具体检测要求而定.)30250    Ni-NTA Agarose (500 ml)    500 ml nickel-charged resin (max. pressure: 2.8 psi)
13343    Blood & Cell Culture DNA Midi Kit    25 QIAGEN Genomic-tip 100/G, QIAGEN Protease, Buffers
74106    RNeasy Mini Kit (250)    250 RNeasy Mini Spin Columns, Collection Tubes (1.5 ml and 2 ml), RNase-free Reagents and Buffers
19075    Buffer AL    216 ml for 1000 preps
19157    QIAGEN Protease (30 AU)    4 x 7.5 Anson units per vial (lyophilized)
19583    24-Well Blocks RB (24)    24-well blocks with 10 ml round-bottom wells, 24 per case
28604    MinElute Gel Extraction Kit (50)    50 MinElute Spin Columns, Buffers, Collection Tubes (2 ml)
34530    Ni-NTA HRP Conjugate    Horseradish-peroxidase-conjugated Ni-NTA (lyophilized, for 500 ml working solution)
34964    Polypropylene Columns (5-ml)    50/pack, 5 ml capacity
9018095    QIAcube Transport Box
9240568    Centrifuge Locator Cylinder, AP96
9240649    Internal BCR, Keyence, BL-601, AP196/98
9240697    Adaptor, Waste Drain, AP
9240680    Spill Tray, Shaker,Modified, AP
9018163    Electronic box, Qsym
9018139    Tray, extractor, Qsym
9018153    Waste drawer, Qsym
9240968    Barcode camera,  sample tracking, Qsym
9240976    PCB, display, sample tracking, Qsym
19419    QIAvac Connecting System    System to connect vacuum manifold with vacuum pump: includes Tray, Waste Bottles, Tubings, Couplings, Valve, Gauge, 24 VacValves
9238605    Centrifuge Motor, plete
9238631    Keypad, foil
9238647    Mechanical lock with optic, left
9238656    Cable, 50-channel
57414    QIAamp MinElute Media Kit (50)
9238253    Syringe Seal
1032393    Stop Solution (1,8ml/2),KG    Description: Stop Solution (1.8m//2), KG
9018958    Master Mix Block, 1x5ml, 4x1.5ml Tubes
9018264    Service Engineer Toolkit, AP
1054578    HiSpeed Plasmid Mega EF Kit (5)
73224    RNeasy Protect Animal Blood Kit (50)
9019132    Gasket - Cartridge, PyroQ96ID
9019139    Cable kit - Power, PyroQ96ID
9019146    Cable kit - Power, PyroQ96MD
9242062    Cover, evaporation trap, IQ/OQ, QS
1068337    Carrier RNA (Poly rA) (310μl)
9241782    QIAgility Demopool Box
9244202    QIAxcel, Basic Agreement    "Repair service delivery: send to Regional Repair Center
Service response time (repair): 7-10 days turnaround time
Periodic inspection/maintenance: yes, 1/agreement year
Inspection/maintenance delivery: not applicable
Cost coverage for Repair Parts : yes, full coverage
Cost coverage for labor: yes, full coverage
Cost coverage for travel: not included
Replacement system (Loaner) provision: not applicable
Transportation cost coverage: yes, full coverage"
9023303    Sensor, induct, drawer SP, Qsym
74881    RNeasy 96 Universal Tissue Kit (4)
4503265    artus CMV RG PCR Kit (96) CE
4555065    artus M.tuberculosis LC PCR Kit (96) CE
4556065    artus Mycobac. diff. LC PCR Kit (96) CE
150090    REPLI-g Human Control Kit    Human control DNA for 25 x 50 μl whole genome amplification reactions
38041    GNA Cartridge (1ml)
1048146    QIAamp DNA Accessory Set B