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抗神经元核抗体2型抗Ri抗体(ANNA-2Ri)ELISA检测试剂盒购买我公试剂盒免费代测提供技术服务,我们服务宗旨诚信经营,确保每个试剂盒质量!欢迎您的选购!齐一试剂盒主营进口ELISA试剂盒,*试剂盒,ELISA检测试剂盒,齐一生物ELISA试剂盒,大鼠ELISA试剂盒,人elisa试剂盒,小鼠elisa试剂盒
产品名称:抗神经元核抗体2型抗Ri抗体(ANNA-2Ri)ELISA检测试剂盒
规格:96T/48T
检测标本:血清,血浆,尿液,胸腹水,脑脊液,细胞培养上清,组织匀浆等
检测方法:ELISA
检测类型:酶联免疫夹心法
运输保存:2~8°保存6个月
齐一生物主要经营进口原装Elisa试剂盒全国:,种类齐全,价格实惠,购买齐一生物产品的新老客户,可享免费代测实验服务,可检测人源,小鼠,大鼠,猪,狗,猴,兔,羊,鸡,牛,鸭子,鱼,植物等各种样本.全程提供.如需其他Elisa试剂盒说明书及报价,可齐一生物.
特点
一、高效、灵敏、特异的抗体;
二、稳定的重复性和可靠性;
三、吸附性能好,空白值低,孔底透明度高的固相载体;
四、适用血清、血浆、组织匀浆液、细胞培养上清液、尿液等等多种标本类型;
五、节省实验经费.
elisa试剂盒回收率是反应待测物在样品分析过程中的损失的程度,损失越少,回收率越高,如果作标液1PPM,就是1毫克/升,而作出标准数据为0.99毫克/升,就是说你的回收率是99%,这个与真实成分有密切的关系,说明方法的准确度.比如水中总无机氯含量测定,样品水中含有无机氯20mg/L,取100mL被测水样品,加入0.1mL浓度为10mg/mL的含无机氯标准样品,测定时忽略体积变化,如果测定出样品中无机氯为2.98mg/L,则认为回收率为99%.
制备方法
包括以下步骤:
1)抗原表位的计算机筛选;
2)抗原的制备;
3)血清的采集;
4)间接ELISA方法的建立;
5)间接ELISA方法的敏感性和特异性验证;
6)试剂盒的稳定性验证;
7)对屠宰场进行临床检测.该方法简单、快速、灵敏度高、特异性强、稳定性合格、重复性好.应用本发明制得的试剂盒能有效检出感染猪的戊型肝炎病毒,适用于大批量发病样本的检测,可用于猪戊肝的快速诊断,并预防、控制猪戊肝病毒的流行和阻断戊肝病毒由猪向人传播.
组成结构
1、血清:操作过程中避免任何细胞刺激.使用不含热原和内毒素的试管.收集血液后,1000×g离心10分钟将血红细胞迅速小心地分离.
2、血浆:EDTA、柠檬酸盐、肝素血浆可用于检测.1000×g离心30分钟去除颗粒.
3、细胞上清液:1000×g离心10分钟去除颗粒和聚合物.
4、组织匀浆:将组织加入适量生理盐水捣碎.1000×g离心10分钟,取上清液.
5、保存:如果样品不立即使用,应将其分成小部分-70℃保存,避免反复冷冻.尽可能的不要使用溶血或高血脂血.如果血清中大量颗粒,检测前先离心或过滤.不要在37℃或更高的温度加热解冻.应在室温下解冻并确保样品均匀地充分解冻.
133280 NeXtalStock TRICINE pH 7.5 (200)
4504005 artus Parvo B19 LC PCR Kit (96) RUO
9240726 Tube Assy, Jar/Pump, AP96/196
9240725 Tube Assy, Fill, AP96/196
9240714 Contact Blade Kit, Capper, AP
L2172 hc2 GC-ID DNA Test PI (CD-ROM)
L2171 hc2 CT-ID HC2 DNA Test PI (CD-ROM)
12945 QIAGEN Plasmid Plus Midi Kit (100) "100 QIAGEN Plasmid Plus Midi Columns, Extender Tubes, Reagents, Buffers, 100 QIAfilter Midi Cartridges
QIAGEN Plasmid Plus Kits enable ultrafast, large-scale plasmid purification of pure plasmid DNA. The use of a vacuum manifold allows purification of up to 12 (Mega and Giga) or up to 24 (Midi and Maxi) samples in parallel with low hands-on needs. Low elution volumes yield highly concentrated plasmid DNA for direct use without ethanol precipitation. QIAGEN Plasmid Plus Kits also feature a novel wash buffer for endotoxin reduction."
9240704 Nipple, Suction Cup, AP
9240689 Jam nut, Shaker Bimba, AP98
330540 Supplemental Microbial qPCR MM (Fluor)
208252 QuantiNova Probe PCR Kit (100)
288107 bactotype Mycoplasma Mg/Ms PCR Kit (480)
1053393 Buffer RLT Plus (220 ml) Please use this for replacement and stand-alone selling
274302 flocktype IBV Ab (2)
274012 flocktype AIV Ab (2)
272702 pigtype PRRSV Ab (2)
273805 pigtype Yersinia Ab (20)
871101 EGFR PCR Kit (25)
9002244 QIAcube(220-240v) Promo, Priority QIAcube (220-240V) Promo Priority includes the QIAcube instrument +promo iPad, installation and extended warranty. Installation covers hardware and software installation of system and provides user with introductory training. Priority package extends warranty period of the system to 2 years. One preventive maintenance visit is included for each year of the warranty; 2 preventive maintenance visits in total. Service package includes all parts, labor and travel costs with a service response time of 48 hours (2 working days). Performance of the installation and service visits are documented on a GMP/GLP-pliant Field Service documents.
9018909 Rotor-Disc 100 Loading Block
9019520 ZLV ADAPTER KIT INVITEK
9020574 LED Assy, Green, 15" Long, DML 3000
9020599 Head Alignment Fixture, DML 3000
9018042 Splash Guard, RCS1
9018190 Tool, arm parallelism, calibration, QIAC
9022614 Ipad 3
9013384 Board, x-main, BR8000
9013248 BGR System Liquid
9013218 ZLV Screws, plastic, M6 (20)
9013217 ZLV Screws, plastic, M5 (20)
9013205 BGR Labhand li+re Digene
9013195 Tubing,vac.trap outlet,autov.BR8000v1.00
132572 NeXtal Stock Jeffamine M-600 (50) 100% v/v O-(2-Aminopropyl)-O'-(2-methoxyethyl)polypropylene 500 - 50mL (5 x 10 ml tube)
132558 NeXtal Stock Lithium sulfate (50) 1M Lithium sulfate monohydrate - 50mL (4 x 12,5 ml tube)
800333 PCR Design
800301 Heterozygote detection, automated "PCR amplification of genomic DNA
QIAGEN DNA template preparation
Sequencing and sequence alignment
Automatic detection of mutations and
heterozygotic positions
Mutation report
Accuracy >99%"
800251 Single Read HT, w/o prep
800151 Basic Read Plates
4514263 artus BK Virus RG PCR Kit (24) CE
4513233 artus HI Virus-1 RG RT-PCR Kit (24) RUO
130710 NeXtal Tubes pHClear II Suite
130707 NeXtal Tubes Anions Suite
120007 Collection Microtubes and Caps Nonsterile polypropylene tubes (1.2 ml), 2304 in packs of 96, and nonsterile polypropylene caps for Collection Microtubes
79216 Buffer RLT 220 ml RNeasy Lysis Buffer
76154 RNAlater TissueProtect Tubes (50x1,5ml) For stabilization of RNA in 50 x 150 mg tissue samples: 50 screw-top tubes containing 1.5 ml RNAlater RNA Stabilization Reagent each
74524 RNeasy Protect Bacteria Mini Kit (50) RNeasy Mini Kit (50) and RNAprotect Bacteria Reagent (2 x 100 ml)
69980 TissueLyser LT Adapter, 12-Tube
19530 Vacuum Regulator For use with QIAvac manifolds
19081 Buffer AW1 242 ml Wash Buffer (1) Concentrate for 1000 spin, 250 midi, or 100 maxi preps
19064 Buffer N3 500 ml Neutralization Buffer
19055 Buffer QC 1000 ml Wash Buffer
13362 Blood & Cell Culture DNA Maxi Kit 10 QIAGEN Genomic-tip 500/G, QIAGEN Protease, Buffers
12843 CompactPrep Plasmid Midi Kit (25) "For ultrafast purification of up to 200 μg or 750 μg Molecular Biology Grade plasmid or cosmid DNA for standard applications
CompactPrep Plasmid Kits enable fast, large-scale plasmid purification in a miniprep format. The use of a vacuum manifold allows 24 samples to be purified in parallel on a single workbench"
4503063 artus CMV LC PCR Kit (24) CE
4502303 artus VZV QS-RGQ Kit (24) RUO
4502163 artus VZV TM PCR Kit (24) CE
990558 Reagent Trough Lid 270 mL (15)
337016 qBiomarker Mutation Assay Control DNA
4502003 artus VZV LC PCR Kit (24) RUO
949016 Autopure 100% Isopropanol (3800 ml)
990393 Reagent Bottles, 30 ml (6)
990392 Rotor Adapter Holder
940054 BioSprint 96 DNA Blood Kit (48)
939018 Deparaffinization solution (50ml)
331451 miScript PreAMP PCR Kit (12)
1070498 Buffer TCL, 2x (8.5 ml),G
1070211 Cramp (1)
981313 Rotor-Disc 100 (300)
981008 PCR Tubes, 0.2 ml (10000)
9012945 Liquid Handling Kit Plato 3000 (500ul)
9012821 Liquid Handling Kit Plato 3301 (1000ul)
133257 NeXtalStock HEPES sod. salt pH 7.6 (200)
330612 RT2 SYBR Green Fluor FAST Mastermix (12)
330521 RT2 SYBR Green ROX qPCR Mastermix (8)
59665 EpiTect Control DNA, unmethylated (100)
9010046 Modul, Incubator, 6 MTP, P3xxx
9010024 Incubator/stacker 4/4 plates Plato 1/3
206544 Type-it HRM PCR Kit (400)
206243 Type-it Microsalite PCR Kit (200) "The Type-it Microsalite PCR Kit is developed for fast and reliable genotypic analysis of humans, animals, plants, and bacteria using
microsalites, STR, or VNTR markers without the need for lengthy optimization procedures
Features
? Reliable microsalite analysis by multiplex PCR
? Microsalite assay development without optimization
? Successful and specific coamplification of all fragments
? Optimized protocols for any detection platform"
9012737 Syringe pump outlet tubing (4.7m)
9012720 Set of Pipet Tubing Plato 3001
9012717 Troughs, 50ml (100 troughs + lids)
133237 NeXtalStock Sodium citrate pH 5.4 (200)
133236 NeXtalStock Sodium citrate pH 5.2 (200)
9002193 QIAcube (230V), PrioPLUS, no PM "QIAcube (230V) Instrument
Installation of QIAcube instrument & Introductory training
3 years coverage on parts, labor and travel
On site service, 48 hr response
No Preventive Maintenance
Priority service provision over non-contract customers
Documented installation & PM report
Unlimited number of repair service"
9002033 Rotor-Gene Q MDx 5plex HRM System
59205 EpiTect Whole Bisulfitome Kit (100)
59124 EpiTect Plus DNA Bisulfite Kit (48)
929551 QX DNA Size Marker FX174/HaeIII (50 μl)
929528 QX Alignment Marker 50 bp/3 kb (1.5 ml)
972807 PyroMark Gold Q96 Reagents (50 x 96) For performing pyrosequncing reaction on the Q96 MD
972442 PyroMark Q96 SNP HFE (96) For detection of variants in HFE gene
133221 NeXtalStock Sodium ac. H2O pH 5.6 (200)
133220 NeXtalStock Sodium ac. H2O pH 5.0(200)
301704 HiPerFect Transfection Reagent (0,5 ml)
301427 Effectene Transfection Reagent (4x1ml) 4 x 1 ml Effectene Reagent, Enhancer, Buffer; for 160 transfections in 60 mm dishes or 640 transfections in 12-well plates
51326 QIAamp DNA Mini QIAcube Kit (240)
9012672 BGR Peripumpe PP-4 Frontbl.gerade (7135)
9002011 Rotor-Gene Q MDx 2Plex HRM no PC
292063 mericon Sheep Kit (24)
9001912 PyroMark Q24 MDx, PrioPLUS "PyroMark Q24 MDx Instrument
Installation of PyroMark Q24 & Introductory training
Warranty extension to 3 yrs
Repair Center service, response 7-10
Loaner available in 2 days*
Annual Inspections (1 onsite PM per yearr)
Priority service provision over non-contract customers
Documented installation & PM report
Unlimited number of repair service
All cost covered (travel, labor, parts)
(In the event of shipping logistic limitation, QIAGEN reserves the option to repair the instrument on-site. Response time 48 Hrs)"
9001910 QIAgility HEPA/UV (w/o PC) PrioPLUS "QIAgility HEPA/UV (w/o PC) Instrument
Installation of QIAgility instrument & Introductory training
Warranty extension to 3 yrs
On site service, 48 hr response
Preventive Maintenance (1 onsite pipetting head service and inspection per year)
Priority service provision over non-contract customers
Documented installation & PM report
Unlimited number of repair service
All cost covered (travel, labor, parts)"
133116 NeXtalStock Sodium thiocyanate (200)
967142 RNeasy 96 BioRobot 9604 Kit (12) For 12 x 96 total and cytoplasmic RNA preps on the BioRobot 9604: 12 RNeasy 96 Plates, Elution Microtubes CL (1.2 ml), Caps, Square-Well Blocks, RNase-free Reagents and Buffers
962141 QIAprep 96 Turbo BioRobot Kit (4) For 4 x 96 high-purity plasmid minipreps, 4 each: TurboFilter 96 and QIAprep 96 Plates; Flat-Bottom Blocks and Lids, Reagents, Buffers, Collection Microtubes (1.2 ml) and Caps, 96-Well Microplates RB and Lids, Tape Pads
960241 QIAGEN Plasmid Plus 96 BioRobot Kit (4)
51304 QIAamp DNA Mini Kit (50) For 50 DNA preps: 50 QIAamp Mini Spin Columns, QIAGEN Proteinase K, Reagents, Buffers, Collection Tubes (2 ml)
978756 PyroMark CpG Assay 96 well (200)
9012712 Z-motor (Plato 7) left
9012696 Set of X-flat band cable (Plato 7)
133239 NeXtalStock Sodium citrate pH 7.0 (200)
133238 NeXtalStock Sodium citrate pH 5.8 (200)
9002127 QIAsymphony RGQ, Priority QIAsymphony RGQ Priority includes the QIAsymphony RGQ instruments, installations and extended warranties. Installation covers hardware and software installation of system and provides user with introductory training. Priority package extends warranty period of the system to 2 years. One preventive maintenance visit is included for each year of the warranty; 2 preventive maintenance visits in total. Service package includes all parts, labor and travel costs with a service response time of 48 hours (2 working days). Performance of the installation and service visits are documented on a GMP/GLP-pliant Field Service documents.
59203 EpiTect Whole Bisulfitome Kit (25)
59164 EpiTect Plus LyseAll Bisulfite Kit (48)
929526 QX Alignment Marker 50 bp/1 kb (1.5 ml)
133218 NeXtalStock MOPS pH 8.0 (200)
301605 RNAiFect Transfection Reagent (1ml) RNAiFect Reagent and buffer, for up to 170 transfections in 24-well plates; up to 500 transfections in 96-well plates