抗磷脂抗体（AplAPA）ELISA检测试剂盒详细说明书请来电，或者在线客服进行索要！齐一生物长期经营ELISA试剂盒及抗体（免费代测）、分子学生物试剂盒、比色法，IFCC推荐法，化学氧化法，速率法等检测试剂盒、细胞菌株、培养基、细胞因子、生化试剂、耗材等生物试剂产品。诚信经营，价格实惠，服务周到，质量保障！提供各种实验代测服务。欢迎新老客户！

抗磷脂抗体（AplAPA）ELISA检测试剂盒产品规格：96T/48T
产品库存：现货
产品价格：询价
使用范围：仅供科研检测,不得用于临床.
保存条件：2-8℃
发货方式：专门快递免费送货上门
产品价格：*,洽谈！
说明书:说明书随货发送,您也可以直接我司在线销售人员索取.全国：代理多种品牌进口原装、分装“ELISA试剂盒".齐一生物科技有限公司作为酶联免疫供应商,凭借*的技术,*的售前,售中,售后免费代测服务,共同铸就高品质的产品.多年专业酶免服务,质量保证,可免费提供代测服务,一周内出结果.欢迎订购.
ELISA试剂盒操作步骤
ELISA试剂盒使用前,将所有试剂充分混匀.不要使液体产生大量的泡沫,以免加样时加入大量的气泡,产生加样上的误差.
根据待测样品数量加上标准品的数量决定所需的板条数.每个标准品和空白孔建议做复孔.每个样品根据自己的数量来定,能使用复孔的尽量做复孔.标本用标本稀释液1：1稀释后加入50ul于反应孔内.
加入稀释好后的标准品50ul于反应孔、加入待测样品50ul于反应孔内.立即加入50ul的生物素标记的抗体.盖上膜板,轻轻振荡混匀,37℃温育1小时.
甩去孔内液体,每孔加满洗涤液,振荡30秒,甩去洗涤液,用吸水纸拍干.重复此操作3次.如果用洗板机洗涤,洗涤次数增加一次.
每孔加入80ul的亲和链酶素-HRP,轻轻振荡混匀,37℃温育30分钟.
甩去孔内液体,每孔加满洗涤液,振荡30秒,甩去洗涤液,用吸水纸拍干.重复此操作3次.如果用洗板机洗涤,洗涤次数增加一次.
每孔加入底物A、B各50ul,轻轻振荡混匀,37℃温育10分钟.避免光照.
取出酶标板,迅速加入50ul终止液,加入终止液后应立即测定结果.
在450nm波长处测定各孔的OD值.
ELISA操作步骤
酶联免疫吸附实验材料,试剂,溶液
1．酶标板,100μltip头,1mlEp管,湿盒
2．包被稀释液（0.05mol/L碳酸钠-碳酸氢钠buffer,pH9.6）
碳酸钠0.15g,碳酸氢钠0.29g,叠氮钠0.02g,加双蒸水100ml,调pH9.6
3.封闭液(5%小牛血清/PBS溶液)
小牛血清5ml,1*PBS(pH7.4)95ml
4.洗涤液(PBST,pH7.4)
NaCl0.8g,KH2PO40.02g,Na2HPO4.12H2O0.29g,KCl0.02g,Tween200.05ml,叠氮钠0.01g,加双蒸水100ml,调pH7.4
5.样本稀释液(PBS,pH7.4)
NaCl0.8g,KH2PO40.02g,Na2HPO4.12H2O0.29g,KCl0.02g,叠氮钠0.01g,加双蒸水100ml,调pH7.4
6.酶标第2抗体(羊抗兔)稀释范围1:5,000-1:100,000
7.底物液(TMB-过氧化氢尿素溶液)
①底物液A:TMB20mg,无水乙醇10ml,加双蒸水100ml.
②底物液B(0.1mol/L柠檬酸-0.2mol/L磷酸二氢钠缓冲液,pH5.0-5.4)
Na2HPO41.46g,柠檬酸0.933g,0.75%过氧化氢尿素0.64ml,加三蒸水100ml,调pH5.0-5.4
③底物A和B按1:1混合即成TMB-过氧化氢尿素溶液.
8.终止液(2mol/LH2SO4溶液)双蒸水200ml,浓硫酸34ml,(缓慢滴加并不断搅拌),加双蒸水300ml
9.0.9%生理盐水

800356    Handling Fee
9015298    Nozzles, M48 (6)
9243775    QIAsymphony AS Custom Protocol Advanced2
9019447    Sensor brd 4 CH temp sensor
9019444    Electrolytic capacitor 8000uF x 63V
9018158    Waste station, waste drawer, Qsym
9018154    Bottle, waste drawer, Qsym
9018135    Sensor, xz, et/hs, Qsym
9018132    Splash guard, extractor, Qsym
9017720    Appl. Pack Investigator for Univ.PF
9017832    Sensor, tip check, QIAC
9017830    Power entry port, QIAC
9017828    Power supply, 230 V, QIAC
9017821    Board, main, QIAC
9017820    Flexcircuit, display, 40 pol. , QIAC
9017812    Board, centrifuge (240V), QIAC
4846024    careUU PCR ASSAY
4861024    careEBV PCR ASSAY II
130906    NeXtal DWBlock MPD Suite
4518265    artus HCV RG RT-PCR Kit (96) CE
130720    NeXtal Tubes JCSG+ Suite
129114    Nuclease-Free Water (10 x 50 ml)
4504065    artus Parvo B19 LC PCR Kit (96) CE
120025    TurboFilter 96 Plates (96)    96 TurboFilter 96 Plates
4505065    artus HAV LC RT-PCR Kit (96) CE
4506063    artus HBV LC PCR Kit (24) CE
75742    RNeasy Fibrous Tissue Midi Kit (10)    10 RNeasy Midi Spin Columns, Collection Tubes (15 ml), Proteinase K, RNase-free DNase I, RNase-free Reagents and Buffers
74181    RNeasy 96 Kit (4)    For 4 x 96 total RNA preps: 4 RNeasy 96 Plates, Elution Microtubes CL (1.2 ml), Caps, RNase-free Reagents and Buffers
28053    MinElute 96 UF PCR Purification Kit (24)    24 MinElute 96 UF PCR Purification Plates
74121    RNeasy Protect Starter Kit    RNAlater RNA Stabilization Reagent (20 ml), 6 RNeasy Mini Spin Columns, 2 RNeasy Midi Spin Columns, Collection Tubes (1.5 and 2 ml), RNase-free Reagents and Buffers
74104    RNeasy Mini Kit (50)    50 RNeasy Mini Spin Columns, Collection Tubes (1.5 ml and 2 ml), RNase-free Reagents and Buffers
72251    TurboCapture 96 mRNA Kit (5)    For rapid and easy mRNA purification in high-throughput applications. The kit provides a fast and simple procedure for purifying mRNA. Up to 96 samples can be processed simultaneously - ideal for high-throughput applications such as pound screening and target validation (e.g. by siRNA screening).
19407    VacConnectors (500)    500 disposable connectors for use with QIAamp spin columns on luer connectors. Should be ordered in conjuntion with 19408 - VacValves (24).
12281    QIAfilter Plasmid Mega Kit (5)    5 QIAGEN-tip 2500, Reagents, Buffers, 5 QIAfilter Mega-Giga Cartridges
12191    QIAGEN Plasmid Giga Kit (5)    5 QIAGEN-tip 10000, Reagents, Buffers
4502103    artus VZV TM PCR Kit (24) RUO
133271    NeXtalStock PIPES pH 7.0 (200)
133270    NeXtalStock Phosphate-citr. pH 5.2 (200)
990512    Filter-Tips, 50 μl (960)
337620    qBiomarker SYBR Green Fluor Mastermix(2)
337011    qBiomarker Somatic Mutations PCR Assays
4500263    artus HSV-1/2 RG PCR Kit (24) CE
939016    Buffer ATL ( 4 x 50 ml),GPR
4500163    artus HSV-1/2 TM PCR Kit (24) CE
1073925    Ni-NTA Superflow (8 x 500 ml),cus,G
9010931    BGR Greifer P7 (alt)
9010858    BGR DT-Abstreifer 8-fach P7xxx D
931036    QIAsymphony Virus/Bact Mini Kit (192)
133248    NeXtalStock Sodium succinate pH6.5 (200)
330603    RT2 SYBR Green FAST Mastermix (24)
929608    QX Pro Separation Buffer (40 ml)
5000-1415    Knob RB671CSKWL Matte, VTX2
5000-1408    Cable Shielded 1/17HP, VTX2
L2170    hc2 CT/GC HC2 DNA Test PI (CD-ROM)
L1971    Rotary Shaker User's Manual
9240705    O-ring Kit, Capper, Spindle, AP
9240693    Actril Sterilant & Test Strips, AP
9243653    Comprehen Valid., Other Extraction Sys    """The Comprehensive Validation Support plan specific to a laboratory's needs will incorporate all of the requirements for the internal validation guidelines from the FBI's Quality Assurance Standards (QAS).

The Comprehensive Validation Support service includes all travel and labor charges for up to 20 days by the QIAGEN Validation Team. Typically, one week will be required to extract, quantify, and amplify the validation study samples and may require multiple individuals. Usually, two weeks is required to analyze the data and one week to write the final report. The expected time period to plete the Comprehensive Validation is six weeks. The actual time may vary depending on resource availability, including laboratory time dedicated to validation activities.

Lab Work – The QIAGEN specialist(s) will travel to the laboratory to conduct the on-site validation lab work. The QIAGEN specialist team will perform and plete all extractions, quantifications, amplfications, and capillary electrophoresis. The laboratory is responsible for providing systems/protocols that may be needed (generally laboratory SOPs, ex. interpretation guidelines). Additionally, the laboratory staff may be required to provide additional data needed for interpretation (ex. quantification/amplification/CE results) during the data review phase of the validation. 

Once the final scope of the validation is determined, QIAGEN will provide a quote for all consumables required for the validation.  Non-QIAGEN chemistry and accessories are not included and should  be purchased to cover the validation experimental design including qPCR, STR, NIST standards, required samples types (blood, semen, buccal samples) and required substrates (swabs, materials, etc).   The Customer is expected to have all materials on-hand 1 week prior to the validation start date.

Data Analysis – The Comprehensive Validation Support plan enpasses data collection (quants, amps, CE) during the on-site visit. The laboratory may be responsible for sharing (typically electronically via ) any validation data requested after the on-site visit. A QIAGEN specialist will plete all of the data analysis produced from the validation study design. This includes qPCR (quant) and STR (amp) analysis and linkage to starting samples.

Validation data CD/flashdrive – QIAGEN will review the validation data and draft a validation summary document, ensuring pliance with the FBI's QAS requirements/standards. QIAGEN will send validation data in an electronic format (/CD/flashdrive) to the laboratory. The laboratory is responsible for final pilation, any format change preferences, review and signoff of the validation report. The documentation provided by QIAGEN will aide in demonstrating pliance with all accreditation guidelines during laboratory audits. Should a hard copy of the validation study be preferred, the laboratory is required to specify prior to the onset of the validation.

Post-validation QIAGEN-to-Laboratory Transfer – Following pletion of the validation and generation of the draft validation summary document, a designated member of the QIAGEN validation team will review the validation with the Lab Manager and the Technical Leader. Following sign off on the validation report, training of the laboratory DNA staff will occur. Refer to the Post-validation Competency Training for additional information. """
386516    Investigator STR GO! Lysis Buffer (200)
208256    QuantiNova Probe PCR Kit (2500)
208254    QuantiNova Probe PCR Kit (500)
180984    GeneRead Adapter I Set 12plex (72)