大肠杆菌脂多糖抗体详细说明书

大肠杆菌脂多糖抗体详细说明书英文名称    E. coli LPS
中文名称    大肠杆菌脂多糖抗体
别    名    Escherichia coli LPS.
研究领域    细胞生物  免疫学  
抗体来源    Rabbit
克隆类型    Polyclonal
交叉反应    Escherichia Coli, O83
产品应用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量    36kDa
性    状    Lyophilized or Liquid
浓    度    1mg/1ml
免 疫 原    KLH conjugated synthetic peptide derived from Escherichia coli O83:H1 str. NRG 857C Lipopolysaccharides (301-331aa)
亚    型    IgG
纯化方法    affinity purified by Protein A
储 存 液    0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
保存条件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
产品介绍    background:
Some form of LPS is a major component of the cell membrane of Gram negative bacteria, contributing greatly to the structural integrity of the bacteria, and protecting the membrane from certain kinds of chemical attack. LPS is an endotoxin, and induces a strong response from normal animal immune systems. LPS function has been under experimental research for several years due to its role in activating many transcriptional factors, which become active after stimulation with LPS. LPS also produces many types of mediators involved in septic shock.

大肠杆菌脂多糖抗体详细说明书Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Antigen: bs-2351P, 0.2ug/100ul 
Primary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; 
Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000; 
TMB(C-0024) staining; 
Read the data in MicroplateReader by 450nm.