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上海现货白介素27(IL-27)检测试剂盒ELISA试剂盒操作步骤
ELISA试剂盒使用前,将所有试剂充分混匀.不要使液体产生大量的泡沫,以免加样时加入大量的气泡,产生加样上的误差.
根据待测样品数量加上标准品的数量决定所需的板条数.每个标准品和空白孔建议做复孔.每个样品根据自己的数量来定,能使用复孔的尽量做复孔.标本用标本稀释液1:1稀释后加入50ul于反应孔内.
加入稀释好后的标准品50ul于反应孔、加入待测样品50ul于反应孔内.立即加入50ul的生物素标记的抗体.盖上膜板,轻轻振荡混匀,37℃温育1小时.
甩去孔内液体,每孔加满洗涤液,振荡30秒,甩去洗涤液,用吸水纸拍干.重复此操作3次.如果用洗板机洗涤,洗涤次数增加一次.
每孔加入80ul的亲和链酶素-HRP,轻轻振荡混匀,37℃温育30分钟.
甩去孔内液体,每孔加满洗涤液,振荡30秒,甩去洗涤液,用吸水纸拍干.重复此操作3次.如果用洗板机洗涤,洗涤次数增加一次.
每孔加入底物A、B各50ul,轻轻振荡混匀,37℃温育10分钟.避免光照.
取出酶标板,迅速加入50ul终止液,加入终止液后应立即测定结果.
在450nm波长处测定各孔的OD值.Pierce PA127246 Adenosine Deaminase (HRP) 1 mg
Pierce PA127247 alpha-1 Acid Glycoprotein (HRP) 1 mg
Pierce PA127248 Pepsin (Biotin) 1 mg
Pierce PA127249 Urease (Biotin) 1 mg
Pierce PA127250 Trypsinogen (Biotin) 1 mg
Pierce PA127251 Transferrin (Biotin) 1 mg
Pierce PA127253 Ribonuclease A (Biotin) 1 mg
Pierce PA127254 Pyruvate Kinase (Biotin) 1 mg
Pierce PA127255 Penicillinase (Biotin) 1 mg
Pierce PA127256 Papain (Biotin) 1 mg
Pierce PA127264 beta Amylase (Biotin) 1 mg
Pierce PA127266 Asparaginase (Biotin) 1 mg
Pierce PA127270 Aldolase (Biotin) 1 mg
Pierce PA127272 Alcohol Dehydrogenase (Biotin) 1 mg
Pierce PA127276 hCG alpha 100 μg
Pierce PA127282 Influenza A 1 ml
Pierce PA127284 Pierce(R) Anti-Apolipoprotein B Rabbit Polyclonal Antibody, 1 ml
Pierce PA127289 Borrelia burgdorferi (HRP) 500 μl
Pierce PA127302 Neisseria gonorrhoeae (FITC) 100 μl
Pierce PA127308 Pierce(R) Anti-Influenza A Goat Polyclonal Antibody, 100 μl
Pierce PA127317 Neisseria gonorrhoeae (HRP) 100 μl
Pierce PA127326 Influenza A M1 100 μl
Pierce PA127328 Herpes Simplex Virus 1&2 (HRP) 100 μl
Pierce PA127331 Chlamydia trachomatis (HRP) 100 μl
Pierce PA127341 Borrelia burgdorferi 100 μl
Pierce PA127357 Influenza A M1 (HRP) 100 μl
Pierce PA127375 E. coli O+K (HRP) 100 μl
Pierce PA127381 Candida albicans (FITC) 100 μl
Pierce PA127382 Cytomegalovirus AD169 (Biotin) 100 μl
Pierce PA127386 Neisseria gonorrhoeae (Biotin) 100 μl
Pierce PA127388 Rubella virions 100 μl
Pierce PA127394 PDGF B 200 μl
Pierce PA127396 Collagen I 100 μl
Pierce PA127397 Collagen I 100 μl
Pierce PA127398 Collagen II 100 μl
Pierce PA127399 Collagen II 100 μl
Pierce PA127409 CCR2 100 μg
Pierce PA127410 RPS16 100 μg
Pierce PA127415 Pierce(R) Anti-Protein A Chicken Polyclonal, 1 mg 1 mg
Pierce PA127477 VMAT2 100 μg
Pierce PA127479 B2M 50 μg
Pierce PA127486 PNLIPRP2 50 μg
Pierce PA127487 B17.2 50 μg
Pierce PA127488 PPP1CC 50 μg