补体片断5b(C5b)ELISA检测试剂盒齐一生物的ELISA试剂盒是您的*选择:每个试剂盒都经过严格的质检控制,包括:板内、板间实验精度,灵敏度,回收率,稳定性等指标的测定。我们提供的96孔板已经预包被好捕获抗体,三步法检测体系简单而直接,可根据450 nm波长的读值迅速计算定量结果。试剂盒中提供的检测抗体能够识别全长靶蛋白的抗原决定簇以及它们的三维结构,使您的检测结果更稳定可靠
补体片断5b(C5b)ELISA检测试剂盒一、高效、灵敏、特异的抗体;
二、稳定的重复性和可靠性;
三、吸附性能好,空白值低,孔底透明度高的固相载体;
四、适用血清、血浆、组织匀浆液、细胞培养上清液、尿液等等多种标本类型;
五、节省实验经费.
elisa试剂盒回收率是反应待测物在样品分析过程中的损失的程度,损失越少,回收率越高,如果作标液1PPM,就是1毫克/升,而作出标准数据为0.99毫克/升,就是说你的回收率是99%,这个与真实成分有密切的关系,说明方法的准确度.比如水中总无机氯含量测定,样品水中含有无机氯20mg/L,取100mL被测水样品,加入0.1mL浓度为10mg/mL的含无机氯标准样品,测定时忽略体积变化,如果测定出样品中无机氯为2.98mg/L,则认为回收率为99%.
制备方法
包括以下步骤:
1)抗原表位的计算机筛选;
2)抗原的制备;
3)血清的采集;
4)间接ELISA方法的建立;
5)间接ELISA方法的敏感性和特异性验证;
6)试剂盒的稳定性验证;
7)对屠宰场进行临床检测.该方法简单、快速、灵敏度高、特异性强、稳定性合格、重复性好.应用本发明制得的试剂盒能有效检出感染猪的戊型肝炎病毒,适用于大批量发病样本的检测,可用于猪戊肝的快速诊断,并预防、控制猪戊肝病毒的流行和阻断戊肝病毒由猪向人传播.
组成结构
1、血清:操作过程中避免任何细胞刺激.使用不含热原和内毒素的试管.收集血液后,1000×g离心10分钟将血红细胞迅速小心地分离.
2、血浆:EDTA、柠檬酸盐、肝素血浆可用于检测.1000×g离心30分钟去除颗粒.
3、细胞上清液:1000×g离心10分钟去除颗粒和聚合物.
4、组织匀浆:将组织加入适量生理盐水捣碎.1000×g离心10分钟,取上清液.
5、保存:如果样品不立即使用,应将其分成小部分-70℃保存,避免反复冷冻.尽可能的不要使用溶血或高血脂血.如果血清中大量颗粒,检测前先离心或过滤.不要在37℃或更高的温度加热解冻.应在室温下解冻并确保样品均匀地充分解冻.
此IBL试剂盒能用于小鼠血清,EDTA血浆,细胞上清中产品定量检测试剂盒成分
1预包被板:抗小鼠白介素-6兔子IgG,亲合纯化96T
2酶标记抗体:(30倍浓缩)HRP标记抗小鼠白介素-6兔子IgG,亲合纯化0.4mLx1
3标准品:重组小鼠白介素-60.5mLx2
4EIA缓冲液:含1%BSA,0.05%吐温20BPS30mLx1
5标记抗体稀释液:含1%BSA,0.05%吐温20BPS12mLx1
6显色剂:TMB底物液15mLx1
7终止液:1N硫酸12mLx1
8浓缩洗涤液:(40倍浓缩)含1%BSA,0.05%吐温20BPS50mLx1操作说明1实验所需器材(但试剂盒没有提供)酶标仪(450nm)微移液管及其吸嘴量筒及烧杯去离子水冰箱(4°C)坐标纸(log/log)吸水纸试管(用于标准品稀释)温育箱(37°C±1°C)洗瓶(用于洗板)一次性试剂管(用于浓缩酶标记抗体和显色剂)
MS IGG1 KPA ITCL NHP FITC 50TST MOPC-21 551954
MS CXCR5 PE MAB 0.1MG 2G8 551959
MS CXCR5 BIOTIN MAB 0.1MG 2G8 551960
MS CXCR5 PURE MAB 0.1MG 2G8 551961
NF-M P-SPEC PURE MAB W/CONT 50UG RNF406 551962
HU TLR4 PURE MAB 0.1MG HTA125 551964
MS CD184 PE MAB 0.1MG 2B11/CXCR4 551966
MS CD184 FITC MAB 0.1MG 2B11/CXCR4 551967
MS CD184 BIOTIN MAB 0.1MG 2B11/CXCR4 551968
MS CD212 BIOTIN MAB 0.1MG 114 551973
MS CD212 PE MAB 0.1MG 114 551974
HU TLR4 BIOTIN MAB 100TST HTA125 551975
RAT IGG1 KPA ITCL NHP PE MAB 50TST R3-34 551979
HU CD4 NHP APC MAB 50TST L200 551980
RAT CD147 PURE MAB 0.1MG OX-47 552012
MS LY-49A BIOTIN MAB 0.1MG JR9-318 552014
MS CD278 PURE MAB 0.5MG 7E.17G9 552015
LUMINOMETER INJECTION CLEANING SOLUTION 552017
HU CD200 PURE MAB 0.1MG MRC OX-104 552023
HU CD172B PURE MAB 0.1MG B4B6 552024
HU CD138 PE MAB 100TST MI15 552026
HU CXCR5 PURE MAB 0.1MG RF8B2 552032
HU TLR1 PURE MAB 0.1MG GD2 552033
FUT-175 (FUTHAN) LYO 5.0MG 552035
MS CD4 APC-CY7 MAB 0.1MG GK1.5 552051
HU CD14 NALE MAB 0.5MG 3-C39 552052
CDK8 PURE MAB 150UG F9-1075 552053
CDK8 PURE MAB 50UG F9-1075 552056
MS LY-6G LY-6C PERCP-CY5.5 0.1MG RB6-8C5 552093
MS CD45R APC-CY7 MAB 0.1MG RA3-6B2 552094
HU CXCR5 BIOTIN MAB 100TST RF8B2 552118
HU CD124 ALP BIOTIN MAB 100TST HIL4R-M57 552120
MS SIGLEC-F PURE MAB 0.1MG E50-2440 552125
MS SIGLEC-F PE MAB 0.1MG E50-2440 552126
HU CD3 NHP PE MAB 50TST SP34-2 552127
MS CD180 PURE MAB 0.5MG RP/14 552128
MS CD180 BIOTIN MAB 0.5MG RP/14 552129
RAT MYELOID LINEAGE PURE MAB 0.1MG OX-82 552130
MS CTLA-4 IGG NALE FUSION PTEIN 0.5MG 552133
HU IFN-GMA ELISPOT KIT 2PLT 552138
MS V DTA 4 TCR FITC MAB 0.1MG GL2 552143
HAM IGG3 KPA ITCL PE MAB 0.1MG E36-239 552144
MS CD278 BIOTIN MAB 0.5MG 7E.17G9 552145
MS CD278 PE MAB 0.2MG 7E.17G9 552146
QIAsoft 4.1.4.8 Servicepack 9016114
Board, YZ6L, master, BR8 AT3/B10 9016048
Software QIAsoft MDx Service Edition 9015913
Fittings (10), M6/2.8 mm, white 9015686
Board, power branching 9015528
Board, operation panel 9015527
Vacuum Pump (100 V), PyroQ24VW 9021234
Skin Panel, Waste Drawer, DCU 9021114
Skin Panel, Lower Center Rear, DCU 9021100
Vial Robot Assist Assembly, DCU 9020996
Kit, calibration, mech. variation, RG3 9020937
Pulley, x idler belt tensioner, C34 9020708
Spindle nut - XY, PyroQ24 9020691
Display, keypad, GSC 9020487
Kit, fuse AS, Qsym 9020390
PM kit, C12 & QIAgility, CG1 9020316
Packaging, foam base, C1x 9020265
Z-pulley, idler ass., 8 ch. gearbox, Cx8 9020234
Belt, Y-axis assembly, Cx8 9020228
Fitting,Peek,10-32,1/16tubing (5) 9239525
Vacuum Pump (110-120 V), PyroQ24VW 9020164
Mikrowin data reduction Software 9232820
Cal. Set for Reader 3 000 12 00 9232807
Manual, user, QS 4.1 (WW) 9236528
#Agreement, Plato 3, PM, 1 yr 9236486
Travel Charge, zone A 9236446
1.0 hr 9236439
Holder, tip rack 2 9011884
Power Supply, 400W, BR8, RD v1.0 9236982
X/Y table, PyroQ96ID 9019142
Camera MX5, PyroQ96ID 9019128
Heating block, PyroQ96ID 9019127
Pin, piercing, EZ1 9019027
Worktable, side wall, right, EZ1xl 9019026
Master Mix Block, 5x5ml Tubes 9018959
Reagent Block, 16x0.2ml/1.5ml PCR/FlatBa 9018952
Adapter, COBAS TaqMan 48 Plates 9018941
Precision Pipetting Calibration Kit 9015617
EZ1 RNA Card 9015590
Cover, back ,type C 9015533
Door Lock Upgrade Kit, BR EZ1 9015520
Tube Holder, STS, 5ml, 16 pos (6) 9014187
RoboVac, Base, BR8 RD, v4.2 9014154
Belt, Y-drive, Mounting Tool, BR8000 9013993
Controller, Z Axis, VRA, DCU 9021177
Output Rack, 96 pos, DCU 9021162
Door Assy, Upper Left, DCU 9021058
Controller, Theta Rotary, DCU 9021013
Belt, X Drive, DCU 9021001
PCB, Pipettor, DCU 9020998
Pip. head, non LLS, 1ch. (EXCHANGE), CC2 9020770
Lid, polycarbonate with UV, C34 9020717
Shipment Box, PyroQ24 9020693
Waste chute, bent, SP, Cabinet Qsym 9020644
Door, waste, AS, Cabinet Qsym 9020630
Power supply, AS, Qsym 9020397
Cable - USB, RGQII 9020346
Pipetting head, 8 ch. (EXCHANGE), CX1 9020337
PCB - Mainboard, RGQII 9020330
Cable - LED, RGQII 9020329