Puromycin dihydrochloride
注：本产品仅用于科研，不可用于临床
生物活性
Puromycin dihydrochloride (CL13900 dihydrochloride), an aminonucleoside antibiotic, inhibits protein synthesis
IC50 & Target
Aminoglycoside
体外研究
(In Vitro)
Puromycin blocks protein synthesis after aminoacyl-sRNA formation, and at the same time it leads to the accumulation of small peptides. Both of these effects appear to be due to the splitting of ribosome-bound peptidyl-sRNA,4 which results in release of incomplete peptide chains.[1].
Puromycin, an analog of the 3' end of aminoacyl-tRNA, causes premature termination of translation by being linked non-specifically to growing polypeptide chains. Puromycin has two modes of inhibitory action. The first is by acting as an acceptor substrate which attacks peptidyl-tRNA in the P site to form a nascent peptide. The second is by competing with aminoacyl-tRNA for binding to the A' site[2].
When used in minimal amounts, puromycin incorporation in neosynthesized proteins reflects directly the rate of mRNA translation in vitro. Puromycin immunodetection is an advantageous alternative to radioactive amino acid labeling. It allows the direct evaluation of translation activity in single cells by immunofluorescence microscopy and in heterogenous populations of cells by fluorescenceactivated cell sorting[3].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量
544.43
性状
Solid
Formula
C22H31Cl2N7O5
CAS 号
58-58-2
来源
Streptomyces alboniger
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
4°C, sealed storage, away from moisture
*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
溶解性数据
H2O : 50 mg/mL (91.84 mM; Need ultrasonic and warming)
DMSO : 50 mg/mL (91.84 mM; Need ultrasonic)
Ethanol : 5 mg/mL (9.18 mM; ultrasonic and warming and heat to 60°C)
| 浓度溶剂体积质量 | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 1.8368 mL | 9.1839 mL | 18.3678 mL |
| 5 mM | 0.3674 mL | 1.8368 mL | 3.6736 mL |
| 10 mM | 0.1837 mL | 0.9184 mL | 1.8368 mL |
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶
- 1.
请依序添加每种溶剂： PBS
Solubility: 100 mg/mL (183.68 mM); Clear solution; Need ultrasonic
- 2.
请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% saline
Solubility: ≥ 2.5 mg/mL (4.59 mM); Clear solution
- 3.
请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in saline)
Solubility: ≥ 2.08 mg/mL (3.82 mM); Clear solution
- 4.
请依序添加每种溶剂： 10% DMSO 90% corn oil
Solubility: ≥ 2.08 mg/mL (3.82 mM); Clear solution
- 5.
请依序添加每种溶剂： 10% EtOH 90% (20% SBE-β-CD in saline)
Solubility: ≥ 0.5 mg/mL (0.92 mM); Clear solution
- 6.
请依序添加每种溶剂： 10% EtOH 90% corn oil
Solubility: 0.5 mg/mL (0.92 mM); Suspended solution; Need ultrasonic
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