克氏锥虫快速检测卡（胶体金法） 返回列表页

克氏锥虫快速检测卡（胶体金法）

名称：克氏锥虫快速检测卡（胶体金法）

规格25T/盒

有效期：24个月1-30度保存

预期用途

锥虫快速检测用于诊断克氏锥虫感染是一种基于特异性多表位重组抗原的快速免疫层析条带分析。该快速检测方法用于定性检测克氏锥虫曲霉菌衍生抗原的血清抗体。仅供研究使用。不用于诊断程序。

The rapid detection of Trypanosoma cruzi infection is a rapid immunochromatographic strip analysis based on specific multi epitope recombinant antigens. This rapid detection method is used to qualitatively detect serum antibodies against Aspergillus derived antigens of Trypanosoma cruzi. For research purposes only. Not used for diagnostic programs.

通过显微镜、血液培养、异种诊断或PCR直接检测血液中的寄生虫具有很高的特异性，可以确认感染的存在（4，5）。然而，这些程序在技术和操作上都要求很高。目前使用的其他方法包括测量针对粗制液的抗体、补体固定、间接血凝和荧光抗体（IFA）。所有这些都缺乏特异性和/或敏感性（6-9）。血清学测试可以检测寄生虫不同发育阶段表达的抗原特异性抗体，非常适合快速简便地诊断该疾病（10-14）。为了改进查加斯病的血清学诊断，我们鉴定并使用了一种来源于不同克鲁兹弓形虫抗原的多表位复合抗原（15，18）。该抗原由总共9个不同的表位组成（15，16）。测试原理此免疫层析测试旨在定性测定抗克鲁兹氏杆菌的血清抗体。此测试可检测克鲁兹氏菌感染者的抗体。该测试基于一种专有的黄金混合物，该混合物含有目标金缀合物及其与血清和全血中存在的抗体结合的能力。一旦结合，金抗体复合物将横向移动，与膜上存在的固定化克鲁兹锥虫衍生蛋白形成复合物，形成测试线。未绑定的黄金将继续向上移动，以绑定形成控制线。当观察到测试线时，测试结果为阳性（见图1）。

Direct detection of parasites in the blood through microscopy, blood culture, heterologous diagnosis, or PCR has high specificity and can confirm the presence of infection (4,5). However, these programs require high technical and operational requirements. Other methods currently used include measuring antibodies against crude liquids, complement fixation, indirect hemagglutination, and fluorescent antibodies (IFA). All of these lack specificity and/or sensitivity (6-9). Serological testing can detect antigen specific antibodies expressed at different developmental stages of parasites, making it very suitable for rapid and simple diagnosis of this disease (10-14). To improve the serological diagnosis of Chagas disease, we identified and used a multi epitope composite antigen (15,18) derived from different antigens of Toxoplasma cruzi. This antigen consists of a total of 9 different epitopes (15, 16). Testing principle: This immunochromatographic test aims to qualitatively determine serum antibodies against Clostridium cruzi. This test can detect antibodies in individuals infected with Clostridia. This test is based on a proprietary gold mixture that contains the target gold conjugate and its ability to bind to antibodies present in serum and whole blood. Once combined, the gold antibody complex will move laterally and form a complex with immobilized Trypanosoma cruzi derived proteins present on the membrane, forming a test line. Unbound gold will continue to move upwards to bind and form a control line. When the test line is observed, the test result is positive