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上海启达生物科技有限公司
上海启达生物科技有限公司
| 产地 | 进口 | 级别 | 其他 |
|---|
人视网膜周细胞（HRPC）
背景介绍/context：
Human Retinal Pericyte Cells，These antibody-free human primary cells were initiated by centrifugal elutriation following dispersal of normal retinal tissue with collagenase and dispase without the use of positive selection of antibodies. 人视网膜周细胞提取于正常视网膜组织，经酶消化纯化，不使用任何阳性抗体筛选，细胞无抗性。
动物种别/Organism：人
组织来源 Tissue and Cell Type：视网膜组织
形态/Morphology：上皮细胞样，贴壁生长
培养基和添加剂/Complete Growth Medium and Culture Conditions：
*培养基：CultureBoost™经典细胞培养基（货号：4N0-500）
气相：空气，95%；二氧化碳，5%。 温度：37摄氏度。
*冻存液：细胞冻存液Cell Freezing Medium（4Z0-705）
人视网膜周细胞（HRPC)出库检测指标：
| Standard Tests | |
| TESTS | RESULTS |
| HIV Serologic Test (donor level HIV AB EIA) | Negative |
| HIV PCR Test (frozen cell pool by CLIA Licensed Clinical Lab) | Negative |
| Hepatitis B (HBV) and Hepatitis C (HCV) PCR Test (at frozen cell level) | Negative |
| Test of frozen cells for Mycoplasma spp. (ATCC method by CLIA Licensed Clinical Lab) | Negative |
| Miscellaneous Tests | |
| TESTS | RESULTS |
| Cytoplasmic alpha-Actinin Filaments | > 80% positive by immunofluorescence |
| Cytoplasmic Desmin Intermediate Filaments | > 80% positive by immunofluorescence |
| Cytoplasmic VWF / Factor VIII | < 0.2% positive by immunofluorescence |
| Cytoplasmic uptake of Di-I-Ac-LDL | < 0.2% positive by immunofluorescence |
| NG2 (Neural/Glial Antigen 2) | >90% positive by immunofluorescence |
| PDGFR-beta (Plaet Derived Growth Factor Receptor beta) | >90% positive by immunofluorescence |
| GFAP (Glial Fibrillary Acidic Protein) | < 1% by immunofluorescence |
| Glutamine Synthetase | < 1% by immunofluorescence |
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