Cell Disruption Application Charts

来源：上海巴崴奥商贸有限公司 2019年02月25日 10:29

Sample	PSI	Passes	Results
Meningicoccal cell paste (4%) in buffer	1000	1	Cooling used to maintain 10C temperature. Complete rupture achieved in one pass.
Mammalian cells	2000	1	Complete rupture of cells and easy separation of parasite within the cell achieved in one pass on laboratory model H-5000.
E. coli (10%) in buffer	6000	3	90% rupture achieved. Results scaled linearly from lab to production.
	10000	1	90% rupture achieved.
	10000	3	90% rupture achieved.
Baker's yeast	20000	10	Cooling used. 95% rupture of yeast cells achieved.
Brewer's yeast (10%) in water	10000	1-10	Cooling used to keep temp below 5C. 35% rupture at 1 pass and 63% in 10 passes.
	20000	1-10	71% rupture at 1 pass and 98% in 10 passes. Yield of enzyme significantly better than that achieved with other processing techniques including French press.

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