抗乙型肝炎病毒e抗体（HBeAb）ELISA检测试剂盒齐一生物主要经营进口原装Elisa试剂盒全国：,种类齐全,价格实惠,购买齐一生物产品的新老客户,可享免费代测实验服务,可检测人源,小鼠,大鼠,猪,狗,猴,兔,羊,鸡,牛,鸭子,鱼,植物等各种样本.全程提供.如需其他Elisa试剂盒说明书及报价,可齐一生物
试剂盒名称：抗乙型肝炎病毒e抗体（HBeAb）ELISA检测试剂盒
规 格：48T/96T
品牌：进口原装/分装/国产
产品的用途：仅供科研究使用！
价格：电议,咨询在线客服,到我司qysw@qiyibio.。齐一生物科技（上海）有限公司提供大量品牌进口原装、以及稳定性强的国产ELISA试剂盒，性价比*欢迎您前来选购！
操作步骤
实验开始前，请提前配置好所有试剂，试剂或样品稀释时，均需混匀，混匀时尽量避免起泡。每次检测都应该做标准曲线。如样品浓度过高时，用样品稀释液进行稀释，以使样品符合试剂盒的检测范围。
1. 加样：分别设空白孔、标准孔、待测样品孔。空白孔加样品稀释液100μl，余孔分别加标准品或待测样品100μl，注意不要有气泡，加样将样品加于酶标板孔底部，尽量不触及孔壁，轻轻晃动混匀，酶标板加上盖或覆膜，37℃反应120分钟。
为保证实验结果有效性，每次实验请使用新的标准品溶液。
2. 弃去液体，甩干，不用洗涤。每孔加生物素标记抗体工作液 100μl（取1μl生物素标记抗体加99μl生物素标记抗体稀释液的比例配制，轻轻混匀，在使用前一小时内配制），37℃,60分钟。
3. 温育60分钟后，弃去孔内液体，甩干，洗板3次，每次浸泡1-2分钟，350μl/每孔，甩干。
4. 每孔加辣根过氧化物酶标记亲和素工作液（同生物素标记抗体工作液） 100μl，37℃，60分钟。
5. 温育60分钟后，弃去孔内液体，甩干，洗板5次，每次浸泡1-2分钟，350μl/每孔，甩干。
6. 依序每孔加底物溶液90μl，37℃避光显色（30分钟内，此时肉眼可见标准品的前3-4孔有明显的梯度蓝色，后3-4孔梯度不明显，即可终止）。
7. 依序每孔加终止溶液50μl，终止反应（此时蓝色立转黄色）。终止液的加入顺序应尽量与底物液的加入顺序相同。为了保证实验结果的准确性，底物反应时间到后应尽快加入终止液。
8. 用酶联仪在450nm波长依序测量各孔的光密度（OD值）。 在加终止液后15分钟以内进行检测。
133186 NeXtalStock Citric acid pH 4.0 (200)
133185 NeXtalStock Citric acid pH 3.8 (200)
9018554 Cover, front, left, EZ1a
9018529 Communicator, Software CD, EZ1a
51206 FlexiGene DNA Kit (250) For purification of DNA from 250 ml whole blood: Buffers, QIAGEN Protease
51106 QIAamp DNA Blood Mini Kit (250) For 250 DNA minipreps: 250 QIAamp Mini Spin Columns, QIAGEN Protease, Reagents, Buffers, Collection Tubes (2 ml)
50214 QIAamp UCP Pathogen Mini Kit (50),G
133102 NeXtalStock Potassium thiocyanate (200)
9015517 Thermocouple
9015469 Motor, M
9001879 QIAxcel, PrioPLUS "QIAxcel Advanced Instrument
Installation of QIAxcel Advanced & Introductory training
Warranty extension to 3 yrs
Repair Center service, response 7-10
Loaner available in 2 days*
Preventive Maintenance (onsite or RRC, 1 PM per year)
Priority service provision over non-contract customers
Documented installation & PM report
Unlimited number of repair service
All cost covered (travel, labor, parts)"
9001876 EZ1 Advanced XL, PrioPLUS "EZ1 Advanced XL Instrument
Installation of EZ1 instrument & Introductory training
Warranty extension to 3 yrs
On site service, 48 hr response
Preventive Maintenance (1 onsite PM per year)
Priority service provision over non-contract customers
Documented installation & PM report
Unlimited number of repair service
All cost covered (travel, labor, parts)"
9001870 Rotor-Gene Q 6plex (PC) PrioPLUS "Rotor-Gene Instrument
Installation of Rotor-Gene & Introductory training
Warranty extension to 3 yrs
Repair Center service, response 7-10
Loaner available in 2 days*
Annual Inspections (1 onsite PM per yearr)
Priority service provision over non-contract customers
Documented installation & PM report
Unlimited number of repair service
All cost covered (travel, labor, parts)"
9012471 Side Panel Assembly, Arm, RCS
958236 MagAttract RNA Cell Mini M48 Kit (192)
9018455 Linear guide, Z axis, QX
9018453 Clip, blue, for tubing (20), QX
133166 NeXtalStock CAPS pH 11.6 (200)
9018430 Key, replacement, grey, QX FA
1068797 Buffer DLB (13mg lyophylized), G
1067930 Buffer ALO (10 ml)
133089 NeXtalStock PEG 6,000 (200)
133075 NeXtalStock PEG 1,500 (200)
38051 LCH Cartridge (1ml)
9015381 Unit, heat/cool, right, M96
204652 QuantiFast Multiplex PCR Kit (80)
204556 QuantiFast ProbeRT-PCR+ROXvial Kit(2000)
805943 REPLI-g Service (100 μg)
290085 mericon Quant. Legionella spp Kit (96)
290043 mericon Campylobacter triple Kit (24)
204356 QuantiFast Probe PCR+ROXVial Kit (2000)
204343 QuantiTect Probe PCR Kit (200) For 200 x 50 ul reactions: 3 x 1.7 ml QuantiTect Probe PCR Master Mix, 2 x 2.0 ml RNase-free water
133290 NeXtalStock TRIS-HCl pH 8.8 (200)
1012829 Effectene Transfection Reagent (100ml) Effectene ONLY!!!
995904 Reagent Container, large, M48 (50)
337840 qBiomarker SYBR ROX FAST Mastermix (2)
9018910 Rotor-Disc 72 Loading Block
9018901 Loading Block 72 x 0.1 mL Tubes
9018706 EZ1 Adv. XL Test Card
9018566 Cable reels, spring-driven, EZ1a
9013003 Linear guide set, V-handler
673013 ipsogen JAK2 MutaScreen Kit (24)
672313 ipsogen PML-RARA bcr3 Kit (24)
672091 ipsogen PML-RARA bcr1 Controls Kit
383215 Investigator Argus X-12 Kit (100)
9243772 QIAsymphony AS Custom Protocol Basic1
380325 Investigator Triplex DSF Kit (100)
9001297 QIAsymphony SP
130919 NeXtal DWBlock Nucleix Suite
4811014 careHCV RT-PCR ASSAY V2
4857021 careMP PCR ASSAY
130825 NeXtal CubicPhase 3w Mo/C 8% μplate (2)
4555205 artus M.tuberculosis RG PCR Kit (96) RUO
130728 Nextal Tubes CubicPhase I Suite
4513263 artus HI Virus-1 RG RT-PCR Kit (24) CE
130719 NeXtal Tubes Nucleix Suite
129834 GelPilot Small Fragment Agarose (500g)
129814 GelPilot LE Agarose (500g)
129046 MaXtract High Density (200 x 1.5 ml)
4504163 artus Parvo B19 TM PCR Kit (24) CE
79000 Oligotex Suspension (0.5) 0.5 ml for mRNA isolation from up to 8 mg of total RNA
76106 RNAlater (250ml) 250 ml RNAlater RNA Stabilization Reagent for stabilization of RNA in 125 x 200 mg tissue samples
75142 RNeasy Midi Kit (10) 10 RNeasy Midi Spin Columns, Collection Tubes (15 ml), RNase-free Reagents and Buffers
27191 QIAprep 96 Turbo Miniprep Kit (4) For 4 x 96 high-purity plasmid minipreps, 4 each: TurboFilter 96 and QIAprep 96 Plates; Flat-Bottom Blocks and Lids, Reagents, Buffers, Collection Microtubes (1.2 ml), Caps
74004 RNeasy Micro Kit (50) 50 RNeasy MinElute Spin Columns, Collection Tubes (1.5 ml and 2 ml), RNase-free DNase I, Carrier RNA, RNase-free Reagents and Buffers
19504 QIAvac 96 Vacuum manifold for processing QIAGEN 96-well plates: includes QIAvac 96 Top Plate, Base, Waste Tray, Plate Holder, Rack of Collection Microtubes (1.2 ml)
19083 Buffer PM (500ml) 500 ml Binding Buffer
9015341 Board, IRB
133046 NeXtalStock Ferric chloride (200)
9012227 Shaker, Tube rack for Plato 7
9012140 Gripper, right, handler, BR8000
9001670 PyroMark Q24 MDx Instrument For methylation and mutation analysis using Pyrosequencing technology in 24 well CE-marked.
9001660 Rotor-Gene Q 6plex System
9001649 Rotor-Gene Q 5plex (w/o PC) PrioPLUS Rotor-Gene Q 5plex (w/o PC) PrioPLUS includes the Rotor-Gene Q system, installation and extended warranty. Installation covers hardware and software installation of system and provides user with on site introductory training. Priority package extends warranty period of the system to 3 years with shipment to Repair Center Service and response turnaround time (repair) 7-10 days. One preventive maintenance visit is included for each year of the warranty; 3 preventive maintenance visits in total. Replacement system (Loaner) provision included (provision time 2-3 days) with transportation cost coverage. The service package includes all parts, labor and travel costs with installation performance and service visits documented on a GMP/GLP-pliant Field Service documents.
9001648 Rotor-Gene Q 5plex (w/o PC) Priority Rotor-Gene Q 5plex (w/o PC) Priority includes the Rotor-Gene Q system, installation and extended warranty. Installation covers hardware and software installation of system and provides user with on site introductory training. Priority package extends warranty period of the system to 2 years with shipment to Repair Center Service has response turnaround time (repair) 7-10 days. One preventive maintenance visit is included for each year of the warranty; 2 preventive maintenance visits in total. Replacement system (Loaner) provision included (provision time 2-3 days) with transportation cost coverage. The service package includes all parts, labor and travel costs with installation performance and service visits documented on a GMP/GLP-pliant Field Service documents.
950263 Final Wash Reagent (VXF) (350 ml)
37561 Qproteome Sialic Glycoprotein Kit
133029 NeXtalStock Cesium chloride (200)
133021 NeXtalStock CABS untit. (200)
9001590 Rotor-Gene Q 6plex Platform
239025 GelPilot 50 bp Ladder (100) Ready-to-use DNA marker; 600 μl (100 lanes), 9 fragments: 50 - 500 bp
231122 QIAGEN PCR Cloning Kit (10) For 10 reactions: 2x Ligation Master Mix (50 ul), pDrive Cloning Vector (0.5 ug), distilled water (1.7 ml)
9015317 Sensor, photo, EE-SX671A
9015305 Block, elution, M48
133148 NeXtalStock BICINE pH 7.5 (200)
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