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单增李斯特菌免疫胶体金试剂盒标本的采集及保存:
1.血清:室温血液自然凝固10-20分钟后,离心20分钟左右(2000-3000转/分)。仔细收集上清。保存 过程 中 如有沉淀形成,应再次离心。
2.血浆:应根据标本的要求选择EDTA、柠檬酸钠或肝素作为抗凝剂,混合10-20分钟后,离心20分钟左右(2000-3000转/分)。仔细收集上清。保存过程中如有沉淀形成,应再次离心。
3.尿液:用无菌管收集。离心20分钟左右(2000-3000转/分)。仔细收集上清。保存过程中如有沉淀形成,应再次离心。胸腹水、脑脊液参照此实行。
4.细胞培养上清:检测分泌性的成份时,用无菌管收集。离心20分钟左右(2000-3000转/ 分)。仔细收集上清。 检测细胞内的成份时,用PBS(PH7.2-7.4)稀释细胞悬液,细胞浓度达到100万/ml左右。通过反复冻融,以 使细胞破坏并 放出细胞内成份。离心20分钟左右(2000-3000转/分)。仔细收集上清。保存过程中如有沉淀形成,应再次离心。
5.组织标本:切割标本后,称取重量。加入一定量的PBS,PH7.4。用液氮迅速冷冻保存备用。标本融化后仍然保持2-8℃的温度。加入一定量的PBS(PH7.4),用手工或匀浆器将标本匀浆充分。离心20分钟左右(2000-3000转/分)。仔细收集上清。分装后一份待检测,其余冷冻备用。
9242546 Probe Holder Assembly, LX200, LC
9242152 Upgrade, CAS1820 vacuum DX/VX "The Upgrade, CAS 1820 vacuum DX/VX upgrades the X-tractorgene to use the improved vacuum system for the QIAxtractor. The upgrade is conducted as an on-site service, performed by a QIAGEN service specialist.
The upgrade allows for the usage of the QIAGEN DX and VX capture plates and the disposable silicon mats.
During the service, the new vacuum system is installed and the software is updated. Please note that due to the software upgrade the instrument will only run the QIAxtractor software applications. Customized run files from the previous version will have to be adapted beforehand for additional fees."
5000-1404 Plate Cartridge 110V, VTX2
5000-1277 Top Housing W LID, DML2
5050-1029 Mouse, PC System spare
5025-1011 Expand 4 Pipettor 240V
9240747 Belt, Reagent Pump 117 groove, AP
9240720 Cable Carrier, Tubing, Dispense Head, AP
9240700 Tubing, Waste, 1 1\4", AP
9243690 DML Luminometer, Repair Fee DML Repair Fee covers Regional Repair Center service for non-service agreement covered DML 2000 and DML 3000 units. Includes labor, parts and shipping materials.
9243652 Comprehen Valid., QuantiPlex/QP Hyres """The Comprehensive Validation Support plan specific to a laboratory's needs will incorporate all of the requirements for the internal validation guidelines from the FBI's Quality Assurance Standards (QAS).
The Comprehensive Validation Support service includes all travel and labor charges for up to 20 days by the QIAGEN Validation Team. Typically, one week will be required to extract, quantify, and amplify the validation study samples and may require multiple individuals. Usually, two weeks is required to analyze the data and one week to write the final report. The expected time period to plete the Comprehensive Validation is six weeks. The actual time may vary depending on resource availability, including laboratory time dedicated to validation activities.
Lab Work – The QIAGEN specialist(s) will travel to the laboratory to conduct the on-site validation lab work. The QIAGEN specialist team will perform and plete all extractions, quantifications, amplfications, and capillary electrophoresis. The laboratory is responsible for providing systems/protocols that may be needed (generally laboratory SOPs, ex. interpretation guidelines). Additionally, the laboratory staff may be required to provide additional data needed for interpretation (ex. quantification/amplification/CE results) during the data review phase of the validation.
Once the final scope of the validation is determined, QIAGEN will provide a quote for all consumables required for the validation. Non-QIAGEN chemistry and accessories are not included and should be purchased to cover the validation experimental design including qPCR, STR, NIST standards, required samples types (blood, semen, buccal samples) and required substrates (swabs, materials, etc). For planning purposes and to ensure a smooth process, the Customer is expected to have all materials on-hand and inventoried one (1) week prior to the validation start date.
Data Analysis – The Comprehensive Validation Support plan enpasses data collection (quants, amps, CE) during the on-site visit. The laboratory may be responsible for sharing (typically electronically via ) any validation data requested after the on-site visit. A QIAGEN specialist will plete all of the data analysis produced from the validation study design. This includes qPCR (quant) and STR (amp) analysis and linkage to starting samples.
Validation data CD/flashdrive – QIAGEN will review the validation data and draft a validation summary document, ensuring pliance with the FBI's QAS requirements/standards. QIAGEN will send validation data in an electronic format (/CD/flashdrive) to the laboratory. The laboratory is responsible for final pilation, any format change preferences, review and signoff of the validation report. The documentation provided by QIAGEN will aide in demonstrating pliance with all accreditation guidelines during laboratory audits. Should a hard copy of the validation study be preferred, the laboratory is required to specify prior to the onset of the validation.
Post-validation QIAGEN-to-Laboratory Transfer – Following pletion of the validation and generation of the draft validation summary document, a designated member of the QIAGEN validation team will review the validation with the Lab Manager and the Technical Leader. Following sign off on the validation report, training of the laboratory DNA staff will occur. Refer to the Post-validation Competency Training for additional information. """
6000-3101 Rapid Capture System
19134 QIAGEN Proteinase K
614033 careHPV Test, SFDA (24-test)
274203 flocktype IBDV Ab (5)
270803 cattletype MAP Ab (5)
280303 cador BVDV RT-PCR Kit (24)
282303 virotype PRRSV RT-PCR Kit (24)
282305 virotype PRRSV RT-PCR Kit (96)
280443 virotype BTV pan/8 RT-PCR Kit (24)
939012 Buffer N2 (2 x 10 ml)
871105 EGFR PCR Kit (100)
800382 Restriction Map
871005 KRAS PCR Kit (100)
9020586 Power Supply, 24V 60W, DML 3000
9013314 Shaker adapter, 192-tube, 5 ml
9013211 Cabling kit, data, BR8000
9011747 Worktable left, BR8000 v1.01
132568 NeXtal Stock Methoxy-PEG 5000 (50) 30% w/v Methoxypolyethylene glycol 5000 - 50mL (4 x 12,5 ml tube)
800311 Custom Primer
HU CD32 PE MAB 100TST FLI8.26 (2003) 550586 美国BD
MS IGM HRP MAB 1.0ML II/41 550588 美国BD
MS IG PE PAB 0.2MG POLY1270 550589 美国BD
MS LY-49I PE MAB 0.1MG YLI-90 550595 美国BD
DFF40/CAD PURE PAB 50UG POLY1260 550603 美国BD
MS IL-1 ALP PURE MAB 0.5MG ALF-161 550604 美国BD
MS IL-1 BTA PURE MAB 0.5MG B122 550605 美国BD
MS IL-1 ALP BIOTIN PAB 0.5MG C1150-27 550606 美国BD
HU KI-67 IHC PURE MAB 1.0ML B56 550609 美国BD
HU TNF OPTEIA KIT 2PLT 550610 美国BD
HU IL-2 OPTEIA KIT 2PLT 550611 美国BD
HU IFN-GMA OPTEIA KIT 2PLT 550612 美国BD
HU IL-10 OPTEIA KIT 2PLT 550613 美国BD
HU IL-4 OPTEIA KIT 2PLT 550614 美国BD
MS IGG1 ITCL BIOTIN MAB 0.25MG MOPC-31C 550615 美国BD
MS IGG1 ITCL FITC MAB 0.25MG MOPC-31C 550616 美国BD
MS IGG1 KPA ITCL PE MAB 0.1MG MOPC-31C 550617 美国BD
MS IGG1 KPA ITCL PE-CY5 0.1MG MOPC-31C 550618 美国BD
HU CD34 NHP PE MAB 50TST 563 550619 美国BD
DCR1 PURE 200UL POLY1267 550622 美国BD
HU CD4 PURE MAB 0.1MG L200 550625 美国BD
DESMIN PURE MAB 50UG RD301 550626 美国BD
MS NK-1.1 APC MAB 0.1MG PK136 550627 美国BD
HU CD4 NHP FITC MAB 50TST L200 550628 美国BD
HU CD4 NHP PE MAB 50TST L200 550630 美国BD
HU CD4 NHP PERCP MAB 50TST L200 550631 美国BD
HU CD195 NHP PE MAB 50TST 3A9 550632 美国BD
HU CD183 NHP PE MAB 50TST 1C6/CXCR3 550633 美国BD
HU CD83 NHP PE MAB 50TST HB15E 550634 美国BD
HAM IG KPA BIOTIN MAB 0.5MG HIG-29 550636 美国BD
HAM IGG1 PURE MAB 0.5MG HIG-632 550637 美国BD
HAM IGG2 PURE MAB 0.5MG HIG-65 550638 美国BD
RAT IL-6 BIOTIN MAB 0.5MG G307-2 550642 美国BD
RAT IL-6 PURE MAB 0.5MG C3-4 550644 美国BD
MS IGG1 KPA ITCL PERCP 0.1MG MOPC-31C 550672 美国BD
MS IGM APC MAB 0.1MG II/41 550676 美国BD
PRNRGC RCPTR AFNTY PURE (P2X7) PAB 200UL 550693 美国BD
PRNRGC RCPTR PURE PAB WITH CONT 50UL 550694 美国BD
RAT V BTA 13 TCR BIOTIN MAB 0.1MG 18B1 550721 美国BD
HU CD212 PURE MAB 0.1MG 2B6/12BTA2 550722 美国BD
HU CD212 PE MAB 100TST 2B6/12BETA2 550723 美国BD
HU CD108 PURE MAB 0.1MG MEM-150 550724 美国BD
HU C5A REC (CD88)IHC PURE 1.0ML C85-2506 550733 美国BD
RAT TNF OPTEIA KIT 2PLT 550734 美国BD
MS ICAD PURE 200UL POLY1266 550736 美国BD
MS CD73 PURE MAB 0.1MG TY/23 550738 美国BD
MS CD73 PE MAB 0.1MG TY/23 550741 美国BD
AKT (PS473) PURE MAB 50UG 104A282 550747 美国BD
鸡透明质酸(HA)检测试剂盒(血清.血浆.组织)
鸡硫酸类肝素(HS)检测试剂盒(血清.血浆.组织)
鸡催乳素(PRL)检测试剂盒(血清.血浆.组织)
鸡白血病病毒抗原(ALV-AG)检测试剂盒(血清.血浆.组织)
鸡促卵泡素(FSH)检测试剂盒(血清.血浆.组织)
鸡促黄体激素(LH)检测试剂盒(血清.血浆.组织)
鸡γ干扰素(IFN-γ)检测试剂盒(血清.血浆.组织)
鸡碳酸酐酶(CA)检测试剂盒(血清.血浆.组织)
鸡抗凝血酶Ⅲ抗体(AT-Ⅲ)检测试剂盒(血清.血浆.组织)
鸡热休克蛋白20(HSP-20)检测试剂盒(血清.血浆.组织)
鸡热休克蛋白60(Hsp-60)检测试剂盒(血清.血浆.组织)
鸡17羟皮质类固醇(17-OHCS)检测试剂盒(血清.血浆.组织)
鸡白介素4(IL-4)检测试剂盒(血清.血浆.组织)
鸡一氧化氮(NO)检测试剂盒(血清.血浆.组织)
鸡单纯疱疹病毒Ⅱ型抗体(HSVⅡ-Ab)检测试剂盒(血清.血浆.组织)
鸡补体3裂解产物(C3SP)检测试剂盒(血清.血浆.组织)
鸡肾上腺髓质素(ADM)检测试剂盒(血清.血浆.组织)
鸡巨噬细胞替代激活相关化学因子1(AmAC-1)检测试剂盒(血清.血浆.组织)
鸡免疫核糖核酸(Irna)检测试剂盒(血清.血浆.组织)
鸡β内酰胺酶(β-lactamase)检测试剂盒(血清.血浆.组织)
鸡流行性乙型脑炎抗体IgG(JEIgG)检测试剂盒(血清.血浆.组织)
鸡α葡萄糖苷酶(a-Glu)检测试剂盒(血清.血浆.组织)
鸡血小板因子4(PF-4CXCL4)检测试剂盒(血清.血浆.组织)
鸡肿瘤坏死因子相关凋亡诱导配体3(TRAIL-R3)检测试剂盒(血清.血浆.组织)
鸡L苯丙氨酸解氨酶(PAL)检测试剂盒(血清.血浆.组织)
鸡烟酰胺腺嘌呤二核苷酸磷酸(NADPH)检测试剂盒(血清.血浆.组织)
鸡B因子(BF)检测试剂盒(血清.血浆.组织)
鸡5核苷酸酶(5-NT)检测试剂盒(血清.血浆.组织)
鸡层连蛋白板层素(LN)检测试剂盒(血清.血浆.组织)
鸡17-酮类固醇(17-KS)检测试剂盒(血清.血浆.组织)
鸡N-乙酰-β-D-氨基葡萄糖苷酶(NAG)检测试剂盒(血清.血浆.组织)
鸡神经胶质纤维酸性蛋白(GFAP)检测试剂盒(血清.血浆.组织)
鸡白介素17(IL-17)检测试剂盒(血清.血浆.组织)
鸡1,3-βD葡葡糖苷酶(1,3-βD-Glu)检测试剂盒(血清.血浆.组织)
鸡可溶性血管内皮细胞蛋白C受体(sEPCR)检测试剂盒(血清.血浆.组织)