人结蛋白(Des)ELISA试剂盒Human desmin,Des ELISAkit齐一生物科技(上海)有限公司以真诚、主动、周到、规范、热情的服务很快得到广大客户的*好评.公司秉承“适应多样化的需求,提供专业化的服务”的经营理念,为客户提供*的服务
人结蛋白(Des)ELISA试剂盒试剂盒组成及试剂配制
1. 酶联板(Assay plate ):一块(96孔).
2. 标准品(Standard):2瓶(冻干品).
3. 样品稀释液(Sample Diluent):1×20ml/瓶.
4. 生物素标记抗体稀释液(Biotin-antibody Diluent):1×10ml/瓶.
5. 辣根过氧化物酶标记亲和素稀释液 (HRP-avidin Diluent):1×10ml/瓶.
6. 生物素标记抗体(Biotin-antibody):1×120μl/瓶(1:100)
7. 辣根过氧化物酶标记亲和素(HRP-avidin):1×120μl/瓶(1:100)
8. 底物溶液(TMB Substrate):1×10ml/瓶.
9. 浓洗涤液(Wash Buffer):1×20ml/瓶,使用时每瓶用蒸馏水稀释25倍.
10. 终止液(Stop Solution):1×10ml/瓶(2N H2SO4).
10、标本的采集及保存
1. 血清:全血标本请于室温放置2小时或4℃隔夜后于1000 x g离心20分钟,取上清即可检测,或将标本放于-20℃或-80℃保存,但应避免反复冻融.
2. 血浆:可用EDTA或肝素作为抗凝剂,标本采集后30分钟内于2 - 8° C 1000 x g离心15分钟,或将标本放于-20℃或-80℃保存,但应避免反复冻融.
3. 细胞培养物上清或其它生物标本:1000 x g离心20分钟,取上清即可检测,或将标本放于-20℃或-80℃保存,但应避免反复冻融.
注:标本溶血会影响zui后检测结果,因此溶血标本不宜进行此项检测
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Anti-TrK A.B.C(Tyrosine kinase,TrkA.B.C) 酪氨酸激酶A.B.C抗体
Anti-Trk B(Tyrosine kinase,Pyk2) 酪氨酸激酶B抗体(神经生长因子受体的一种)
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Anti-Trk C(Tyrosine kinase,TrkC) 酪氨酸激酶C抗体(神经生长因子受体的一种)
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