背景介绍/context:
Human Retinal Pericyte Cells,These antibody-free human primary cells were initiated by centrifugal elutriation following dispersal of normal retinal tissue with collagenase and dispase without the use of positive selection of antibodies. 人视网膜周细胞提取于正常视网膜组织,经酶消化纯化,不使用任何阳性抗体筛选,细胞无抗性。
动物种别/Organism:人
组织来源 Tissue and Cell Type:视网膜组织
形态/Morphology:上皮细胞样,贴壁生长
培养基和添加剂/Complete Growth Medium and Culture Conditions:
*培养基:CultureBoost™经典细胞培养基(货号:4N0-500)
气相:空气,95%;二氧化碳,5%。 温度:37摄氏度。
*冻存液:细胞冻存液Cell Freezing Medium(4Z0-705)
人视网膜周细胞(HRPC)出库检测指标:
Standard Tests | |
TESTS | RESULTS |
HIV Serologic Test (donor level HIV AB EIA) | Negative |
HIV PCR Test (frozen cell pool by CLIA Licensed Clinical Lab) | Negative |
Hepatitis B (HBV) and Hepatitis C (HCV) PCR Test (at frozen cell level) | Negative |
Test of frozen cells for Mycoplasma spp. (ATCC method by CLIA Licensed Clinical Lab) | Negative |
Miscellaneous Tests | |
TESTS | RESULTS |
Cytoplasmic alpha-Actinin Filaments | > 80% positive by immunofluorescence |
Cytoplasmic Desmin Intermediate Filaments | > 80% positive by immunofluorescence |
Cytoplasmic VWF / Factor VIII | < 0.2% positive by immunofluorescence |
Cytoplasmic uptake of Di-I-Ac-LDL | < 0.2% positive by immunofluorescence |
NG2 (Neural/Glial Antigen 2) | >90% positive by immunofluorescence |
PDGFR-beta (Plaet Derived Growth Factor Receptor beta) | >90% positive by immunofluorescence |
GFAP (Glial Fibrillary Acidic Protein) | < 1% by immunofluorescence |
Glutamine Synthetase | < 1% by immunofluorescence |
注意事项:此产品仅供科研使用