背景介绍/context:
Primary Human Aortic Endothelial Cells,These antibody-free human primary cells were initiated from normal human descending aorta (aortic arch to renal artery) without the use of positive selection of antibodies. 人主动脉内皮细胞提取于正常人降主动脉(主动脉弓至肾动脉),不经阳性抗体筛选,细胞无抗性。
动物种别/Organism 人
组织来源 Tissue and Cell Type:主动脉
形态/Morphology :小石子路状,贴壁生长
培养基和添加剂/Complete Growth Medium and Culture Conditions:
*培养基:CultureBoost™经典细胞培养基(货号:4N0-500)
气相:空气,95%;二氧化碳,5%。 温度:37摄氏度。
*冻存液:细胞冻存液Cell Freezing Medium(4Z0-705)
人主动脉内皮细胞(HAEC)细胞出库检测指标:
Standard Tests | |
TESTS | RESULTS |
HIV Serologic Test (donor level HIV AB EIA) | Negative |
HIV PCR Test (frozen cell pool by CLIA Licensed Clinical Lab) | Negative |
Test of frozen cells for Mycoplasma spp. (ATCC method by CLIA Licensed Clinical Lab) | Negative |
Miscellaneous Tests | |
TESTS | RESULTS |
Inducible expression of CD 62E (E-Selectin) | > 90% positive by immunofluorescence |
Cytoplasmic VWF / Factor VIII | > 95% positive by immunofluorescence |
Cytoplasmic uptake of Di-I-Ac-LDL | > 95% positive by immunofluorescence |
注意事项:此产品仅供科研使用