SMCC
SMCC 是一种蛋白质交联剂。SMCC 接合抗原耦合脾脏细胞来诱导抗原特异性免疫反应。
生物活性
SMCC is a protein crosslinker. SMCC-conjugated antigen coupled spleen cells to induce antigen-specific immune responses[1].
体外研究(In Vitro)
SMCC or sulfo-SMCC can use to conjugate keyhole limpet hemocyanin (KLH) and ovalbumin (OVA). In the the conjugation system, 300 μl KLH or OVA (3 mg) and 30 μl SMCC or Sulfo-SMCC (the final concentration of 0.1 mM) are added in PBS with final volume of 3 ml[1].
体内研究(In Vivo)
Using the SMCC-conjugation method is able to induce potent immune responses to whole protein or peptide antigens by injection of antigen-coupled syngeneic splenic mononuclear cells in mice[1].
分子量：334.32
性状：Solid
Formula：C16H18N2O6
CAS 号：64987-85-5
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
4°C, sealed storage, away from moisture and light
*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
溶解性数据
DMSO : 25 mg/mL (74.78 mM; Need ultrasonic)
H2O : < 0.1 mg/mL (ultrasonic) (insoluble)
| 浓度溶剂体积质量 | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 2.9911 mL | 14.9557 mL | 29.9115 mL |
| 5 mM | 0.5982 mL | 2.9911 mL | 5.9823 mL |
| 10 mM | 0.2991 mL | 1.4956 mL | 2.9911 mL |
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶
- 1.
请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% saline
Solubility: ≥ 2.5 mg/mL (7.48 mM); Clear solution
- 2.
请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in saline)
Solubility: ≥ 2.5 mg/mL (7.48 mM); Clear solution
- 3.
请依序添加每种溶剂： 10% DMSO 90% corn oil
Solubility: ≥ 2.5 mg/mL (7.48 mM); Clear solution
参考文献
[1]. Guo Y, et al. Potent antigen-specific immune response induced by infusion of spleen cells coupled with succinimidyl-4-(N-maleimidomethyl cyclohexane)-1-carboxylate (SMCC) conjugated antigens. Int Immunopharmacol. 2016 Feb;31:158-68. [Content Brief]
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