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Nicotinamide (烟酰an)

参考价	￥450.00

参考价

￥450.00

具体成交价以合同协议为准

公司名称杭州昊鑫生物科技股份有限公司
品牌MCE
型号HY-B0150
所在地杭州市
厂商性质代理商
更新时间2024/6/24 15:20:58
访问次数153

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烟酰an

规格

450.00元

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杭州昊鑫生物科技股份有限公司成立于2009年初，是一家面向生命科学领域，从事科研机构、高校、院所及生产企业所需科研试剂、耗材，仪器销售和服务面向全国。

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产品简介在线询价

CAS号	98-92-0	产地	国产
规格	1g	级别	化工级

Nicotinamide 是wei生素 B3 或烟suan的一种形式，可抑制 SIRT2 的体外活性，其 EC50 值为 2 μM。

Nicotinamide (烟酰an) 产品信息

Nicotinamide (Synonyms: 烟酰an; Niacinamide; Nicotinic acid amide)

Nicotinamide 是wei生素 B3 或烟suan的一种形式，可抑制 SIRT2 的体外活性，其 EC₅₀ 值为 2 μM。 Nicotinamide 可抑制 90% 的黑色素瘤细胞数量，并增加细胞内 NAD₊、ATP、ROS 水平。Nicotinamide 抑制黑色素瘤小鼠的肿瘤生长并提高生存率，可用于黑色素瘤等皮肤ai相关的研究。

生物活性

Nicotinamide is a form of vitamin B3 or niacin that inhibits sirtuin 2 (SIRT2) activity in vitro, with an EC₅₀ of 2 μM. Nicotinamide inhibits up to 90% melanoma cell number and increases cellular NAD₊, ATP, ROS levels. Nicotinamide inhibts tumor growth in vivo and improves survival of melanoma-bearing mice, which can be used for the research of skin cancers such as melanoma^[1].

体外研究(In Vitro)

Nicotinamide (0-50 mM, 24/48 h ) reduces cell number in a time-dependent and dose-dependent manner in A375 and SK-MEL-28 cells^[1].

Nicotinamide (10-50 mM, 24 h ) makes A375 cells undergo accumulation in G1 phase, reduction in S phase, and increase inthe sub-G1 (apoptosis) phase^[1].
Nicotinamide (1-50 mM, 6 h ) increases NAD₊, ATP and ROS levels in A375 and SK-MEL-28 cells^[1].
Nicotinamide (0.01-20 mM, 1 h) inhibits purified SIRT2 enzymatic activity in vitro with an EC₅₀ of 2 μM^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	A375, SK-MEL-28, mouse B16-F10 cell
Concentration:	0, 1, 20, 50 mM
Incubation Time:
Result:	24 h, 48 h

体内研究(In Vivo)

Nicotinamide (Intraperitoneal injection, 1500 and 1800 mg/Kg, 5 days per week) inhibits tumor growth in murine metastatic melanoma model^[1].

Nicotinamide (Intraperitoneal injection, 1800 mg/Kg, once a day, murine metastatic melanoma model) affects IFN-γ (a key mediator of cell-mediated anti-tumor immunity), increases the plasma levels of eotaxin and IL-5, reduces the plasma levels of IL-3, IL-12, RANTES and IL-10^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	C57BL/6 mice (subcutaneous injected with B16-F10 cells)^[1]
Dosage:	1000, 1500, 1800 mg/Kg.
Administration:	Intraperitoneal injection, 5 days per week (followed by 2-day rest) or once a day.
Result:	Inhibited tumor growth at 1500 and 1800 mg/Kg, and had no effect on the body weight. Increased the frequency of IFN-γ producing cells and modulated the protein levels of cytokines and chemokines in the plasma of tumor-bearing mice.

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase
NCT04910061	Seneque SA\|Dicentra Inc.\|LGD	Healthy Volunteers	August 5, 2021	Not Applicable
NCT05258474	University Hospital Schleswig-Holstein	Safety Issues\|Pharmacokinetic	December 4, 2020	Phase 1
NCT05194397	Children´s Hospital of Philadelphia\|National Institutes of Health (NIH)\|City of Hope Medical Center\|St. Jude Children´s Research Hospital\|National Cancer Institute (NCI)\|University of Pennsylvania	Acute Lymphoblastic Leukemia in Remission\|Cancer Survivors	November 18, 2022	Phase 2

分子量：122.12

性状：Solid

Formula：C₆H₆N₂O

CAS 号：98-92-0

中文名称：烟酰an

运输条件：Room temperature in continental US; may vary elsewhere.

储存方式：

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性数据

In Vitro:

DMSO : 50 mg/mL (409.43 mM; Need ultrasonic)

H₂O : ≥ 50 mg/mL (409.43 mM)

* "≥" means soluble, but saturation unknown.

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	8.1887 mL	40.9433 mL	81.8867 mL
5 mM	1.6377 mL	8.1887 mL	16.3773 mL
10 mM	0.8189 mL	4.0943 mL	8.1887 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： PBS
Solubility: 100 mg/mL (818.87 mM); Clear solution; Need ultrasonic
2.

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% saline
Solubility: ≥ 2.5 mg/mL (20.47 mM); Clear solution
3.

请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in saline)
Solubility: ≥ 2.5 mg/mL (20.47 mM); Clear solution
4.

请依序添加每种溶剂： 10% DMSO 90% corn oil
Solubility: ≥ 2.5 mg/mL (20.47 mM); Clear solution