IL-6 蛋白, Human

IL-6 蛋白, Human 有效诱导肝细胞合成急性期蛋白，参与炎症反应。

描述

IL-6 Protein, Human is a potent inducer of the synthesis of acute phase proteins in adult human hepatocytes.

研究背景

Interleukin-6 (IL-6) is a 26-kD protein released by fibroblasts, T lymphocytes, endothelial cells, monocytes, and keratinocytes during inflammatory responses. IL-6 has pluripotent activities, including the stimulation of the monocytic lineage, the induction of megakaryopoiesis and the hepatic acute phase response, as well as the modulation of T- and B-cell responses. IL-6 is also capable of mediating direct and indirect tumor cell destruction and that it is potentially important for the immunotherapy of cancer^[1. Recombinant human interleukin-6 (IL-6/BSF-2/HSF) regulates the synthesis of acute phase proteins in human hepatocytes[2].

生物活性

The ED₅₀ is <1.0 ng/mL as measured by TF-1 cells, corresponding to a specific activity of 1× 10⁶ units/mg.

种属：Human

表达系统：E. coli

标签：Tag Free

蛋白编号：P05231 (P29-M212)

基因 ID：3569  [NCBI]

同用名：rHuIL-6; BSF-2; CDF; Hybridoma growth factor; IFN-beta-2

氨基酸序列

PVPPGEDSKDVAAPHRQPLTSSERIDKQIRYILDGISALRKETCNKSNMCESSKEALAENNLNLPKMAEKDGCFQSGFNEETCLVKIITGLLEFEVYLEYLQNRFESSEEQARAVQMSTKVLIQFLQKKAKNLDAITTPDPTTNASLLTKLQAQNQWLQDMTTHLILRSFKEFLQSSLRALRQM

分子量：Approximately 21,000 Da

纯度：Greater than 95% as determined by reducing SDS-PAGE.

性状：Lyophilized powder.

组分：Lyophilized after extensive dialysis against 20 mM HAc-NaAc, pH 5.0, 150 mM NaAc buffer.

复溶方法：It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

保存条件 & 期限

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

参考文献

• [1]. Nieken J, et al. Recombinant human interleukin-6 induces a rapid and reversible anemia in cancer patients. Blood. 1995 Aug 1;86(3):900-5.  [Content Brief]

  [2]. Castell JV, et al. Recombinant human interleukin-6 (IL-6/BSF-2/HSF) regulates the synthesis of acute phase proteins in human hepatocytes. FEBS Lett. 1988 May 23;232(2):347-50.  [Content Brief]

注：产品仅用于科研