Texas Red (Synonyms: 磺基罗丹明 101; Sulforhodamine 101; SR101)
Texas Red (Sulforhodamine 101) 是一种两性罗丹明红色荧光染料 (excitation/emission: 586/605 nm)。Texas Red 被广用于研究神经元形态，并作为星形胶质细胞的细胞类型选择性荧光标记物，在体内和切片制备中都有应用。
生物活性
Texas Red (Sulforhodamine 101) is an amphoteric rhodamine red fluorescent dye (excitation/emission: 586/605 nm). Texas Red is used extensively for investigating neuronal morphology and acts as a cell type-selective fluorescent marker of astrocytes both in vivo and in slice preparations[1].
体外研究(In Vitro)
Texas Red (Sulforhodamine 101) does not label astrocytes in brainstem slices as strong and specific as in the hippocampus or cortex. To minimize excitatory side effects, the concentration of Texas Red has to be kept as low as possible or the labeling procedure can be performed after the actual experiment[1].
体内研究(In Vivo)
In vivo, epileptic activity can be induced by intra-hippocampal injection of small volumes of 10 μM Texas Red or topical application of 100 μM[1].
分子量：606.71
Formula：C31H30N2O7S2
CAS 号：60311-02-6
中文名称：磺基罗丹明101
运输条件：Room temperature in continental US; may vary elsewhere.
储存方式：
-20°C, protect from light
*该产品在溶液状态不稳定，建议您现用现配，即刻使用。
溶解性数据
In Vitro:
DMSO : 50 mg/mL (82.41 mM; Need ultrasonic)
H2O : 8.33 mg/mL (13.73 mM; Need ultrasonic)
| 浓度溶剂体积质量 | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 1.6482 mL | 8.2412 mL | 16.4823 mL |
| 5 mM | 0.3296 mL | 1.6482 mL | 3.2965 mL |
| 10 mM | 0.1648 mL | 0.8241 mL | 1.6482 mL |
请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液；该产品在溶液状态不稳定，建议您现用现配，即刻使用。
以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶
- 1.
请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% saline
Solubility: 2.08 mg/mL (3.43 mM); Suspended solution; Need ultrasonic
- 2.
请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in saline)
Solubility: 2.08 mg/mL (3.43 mM); Suspended solution; Need ultrasonic
参考文献
[1]. J Kang, et al. Sulforhodamine 101 induces long-term potentiation of intrinsic excitability and synaptic efficacy in hippocampal CA1 pyramidal neurons. Neuroscience. 2010 Sep 15;169(4):1601-9. [Content Brief]
注：产品仅用于科研
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