Leupeptin hemisulfate (Synonyms: 亮肽素)
Leupeptin hemisulfate 是一种可透过膜的巯基蛋白酶抑制剂，可抑制 Cathepsin B, Cathepsin H 和 Cathepsin L，并损害两性溶酶体的融合。Leupeptin hemisulfate 也具有抗yan作用。
生物活性
Leupeptin hemisulfate is a membrane-permeable thiol protease inhibitor that inhibits Cathepsin B, Cathepsin H and Cathepsin L, and also impairs amphisome-lysosome fusion[1]. Leupeptin hemisulfate also exhibits anti-inflammatory effect[2]
IC50 & Target：Cathepsin[1]
体内研究(In Vivo)
Leupeptin (0-36 mg/kg; intraperitoneal injection; for 4 hours; C57BL/6NCrl male mice) is well tolerated by the animals and produces a strong, dose-dependent increase in LC3b-II in both the total tissue extracts and the lysosome and autophagosome-enriched pellet fraction
| Animal Model: | C57BL/6NCrl male mice (6-8 weeks old, 20-25 g)[1] |
| Dosage: | 0 mg/kg, 9 mg/kg, 18 mg/kg, 36 mg/kg |
| Administration: | Intraperitoneal injection; for 4 hours |
| Result: | Promoted the accumulation of LC3b-II in mouse liver. |
分子量：475.59
Formula：C20H38N6O4.1/2H2SO4
CAS 号：103476-89-7
equence Shortening：Ac-LLR-CHO
中文名称：亮肽素
运输条件：Room temperature in continental US; may vary elsewhere.
储存方式：
-20°C, sealed storage, away from moisture
*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
溶解性数据
H2O : 83.33 mg/mL (175.21 mM; ultrasonic and warming and heat to 60°C)
| 浓度溶剂体积质量 | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 2.1027 mL | 10.5133 mL | 21.0265 mL |
| 5 mM | 0.4205 mL | 2.1027 mL | 4.2053 mL |
| 10 mM | 0.2103 mL | 1.0513 mL | 2.1027 mL |
请根据产品在不同溶剂中的溶解度选择合
参考文献
[1]. Haspel J, et al. Characterization of macroautophagic flux in vivo using a leupeptin-based assay. Autophagy. 2011 Jun;7(6):629-42.
[2]. Aoyagi T, et al. Biological activities of leupeptins. J Antibiot (Tokyo). 1969 Nov;22(11):558-68.
注：产品仅用于科研
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