KDR人重组蛋白

蛋白名称:KDR人重组蛋白，10012-H02H
蛋白构建:A DNA sequence encoding the extracellular domain of human VEGFR2 (NP_002244.1) (Met 1-Glu 764) was fused with the Fc region of human IgG1 at the C-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
蛋白活性:

1. Immobilized  Human VEGF121 (Cat:10008-HNAH) at 2 μg/mL (100 μL/well) can bind Human VEGFR2/KDR (ECD, hFc Tag)(Cat:10012-H02H), the EC50 of Human VEGFR2/KDR (ECD, hFc Tag)(Cat:10012-H02H) is 6-48 ng/mL. 

2. Measured by its ability to inhibit the VEGF-dependent proliferation of human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is typically 20-120 ng/mL in the presence of 10 ng/mL recombinant human VEGF165.

蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Ala 20
蛋白分子量:The recombinant human VEGFR2/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 979 amino acids and predicts a molecular mass of 109 kDa. The apparent molecular mass of human VEGFR2/Fc monomer is approximatly 150-160 KDa in SDS-PAGE under reducing conditions due to glycosylation.
蛋白NP号:NP_002244.1
蛋白氨基酸序列:Met1-Glu764
蛋白标签:C-human IgG1-Fc

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