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分类名称 : 代谢产物分析 产品介绍 :

测定方法   Bradford比色法（595nm）定量测定总蛋白的浓度。

产品说明   蛋白质被称为“生命的物质基础”，它是生命科学领域zui重要的大分子之一。蛋白质由多肽氨基酸组成，它在生物体内发挥着各种关键的作用。蛋白质定量检测是生命科学家常用的方法。简单、直接、自动化的蛋白质浓度检测方法是zui为理想的。本公司生产的蛋白质测试法是基于改进的考马斯蓝G的方法。显色剂与蛋白质经过复杂反应形成蓝色，其颜色强度（595nm）与样品中的蛋白质浓度成正比。优化配方大幅减少了原样品中物质的干扰以及对多肽的更为敏感。 

产品用途    直接检测样品蛋白质的总含量。

产品特点    灵敏、准确：用10 mL样品。测试范围 0.06 – 1.0 mg/mL 蛋白质.简单、高通量： 测试过程仅需添加工作试剂及读取吸光度。易于自动化高通量测试，每天可检测几千份样品。低干扰:葡萄糖、维生素、氨基酸、DNA、 RNA、盐、 EDTA (< 12 mM) 、 苯酚 (< 50 mM) 、 尿素 (< 0.6 M) 、表面活性剂 (< 0.1%) 和SDS (< 0.1% SDS)均不影响测试。多功能性: 测试可用96孔板或比色皿进行。

储藏条件：室温运输 。试剂和标准品分别在室温和 -20°C 下保存。

Description   The protein is known as the "building blocks of life" and is one of the most important macromolecules in life science. Proteins are polypeptides made up of amino acids and play various key roles in all aspects of biology. Protein quantitation is a very common practice for life scientists. Simple, direct and automation-ready procedures for measuring protein concentration are very desirable. our QuantiChrom^TM protein assay kit is based on an improved Coomassie Blue G method. The dye forms a blue complex specifically with protein, and the intensity of color, measured at 595nm, is directly proportional to the protein concentration in the sample. The optimized formulation substantially reduces interference by substances in the raw samples and exhibits increased sensitivity towards peptides. 

Applications   Direct Assays: total protein concentration.

Key features     Sensitive and accurate. Use 10 mL samples. Detection range 0.06 – 1.0 mg /mL protein in 96-well plate assay.Simple and high-throughput. The “mix-and-read” procedure involves addition of a single working reagent and reading the optical density. Can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day.Low interference. Glucose, Tris, vitamins, and amino acids, DNA, RNA, salts, EDTA (< 12 mM), phenol (< 50 mM), urea (< 0.6 M), Triton (< 0.1%) and SDS (< 0.1% SDS) do not interfere in the assay.Versatility: assays can be executed in 96-well plate or cuvet.

Storage conditions. The kit is shipped at room temperature. Store the reagent at room temperature and standard at -20°C, respectively. Shelf life of at least 6 months (see expiry dates on labels).

编号：QCPR-048,QCPR-500

测试：48T,500T