说明书下载ENH3.rar

分类名称 : 高效药物筛选,代谢产物分析 产品介绍 :

测定方法    比色法(340nm)或荧光法(450/360nm)测定氨浓度

产品说明   对生命机体来说氨(NH₃)或铵离子形式(NH₄⁺)是氮的重要来源。它是通过氨基酸代谢合成，当呈现高浓度时则有毒性。在肝脏，氨通过尿素循环转换成尿素。血液中氨浓度升高(高血氨症)多见于肝功能障碍(肝硬化)患者，而氨浓度低下则与尿素循环酶(如鸟氨酸转移酶)障碍相关。简单，直接，自动化的氨浓度检测手段在科研和药物开发领域是很受欢迎的。博世生物技术的氨浓度检测试剂盒是直接检测NH₃和NH₄⁺的浓度。检测中，NADH在氨、戊二酸和谷氨酸脱氢酶的参与下被转换为NAD⁺ 。在340 nm处光密度或在lem/ex  =  450/360nm处荧光强度与样品中氨的浓度成正比。

产品特点   灵敏度高、线性范围宽: 仅用20 mL的样本。线性检测范围24-1000 mM氨.操作简单: 简单的“混合-检测” 操作, 可在30分钟内检测氨浓度。

适用范围   直接检测：生物样品(如血清，血浆，尿液， 唾液，细胞培养等)中的氨。

贮存条件:  该试剂盒应冰运, 所有试剂均于-20°C保存。 

Description    Ammonia (NH₃) or its ion form ammonium (NH₄⁺) is an important source of nitrogen for living systems. It is synthesized through amino acid metabolism and is toxic when present at high concentrations. In the liver, ammonia is converted to urea through the urea cycle. Elevated levels of ammonia in the blood (hyperammonemia) have been found in liver dysfunction (cirrhosis), while hypoammonemia has been associated with defects in the urea cycle enzymes (e.g. ornithine transcarbamylase).Simple, direct and automation-ready procedures for measuring NH₃ are popular in research and drug discovery. our  ammonia assay is designed to directly measure NH₃ and NH₄⁺. In this assay, NADH is converted to NAD⁺ in the presence of NH₃, ketoglutarate and glutamate dehydrogenase. The decrease in optical density at 340 nm or fluorescence intensity at l_em/ex = 450/360 nm is directly proportionate to the NH₃ concentration in the sample.

Key features   High sensitivity and wide linear range. Use 20 mL sample. Linear detection range 24 to 1000 mM ammonia.Homogeneous and simple procedure. Simple “mix-and-measure” procedure allows reliable quantitation of NH₃ within 30 minutes.

Applications    Direct Assays: NH₃ in biological samples (e.g. serum, plasma, urine, saliva, cell culture etc).

Storage conditions. The kit is shipped chilled.  Store all components at -20°C. Shelf life of at least 3 months.

编号：ENH3-048，ENH3-100

测试：48T,100T