齐一生物铁蛋白(FE)ELISA检测试剂盒齐一生物的ELISA试剂盒是您的*选择:每个试剂盒都经过严格的质检控制,包括:板内、板间实验精度,灵敏度,回收率,稳定性等指标的测定。我们提供的96孔板已经预包被好捕获抗体,三步法检测体系简单而直接,可根据450 nm波长的读值迅速计算定量结果。试剂盒中提供的检测抗体能够识别全长靶蛋白的抗原决定簇以及它们的三维结构,使您的检测结果更稳定可靠
齐一生物铁蛋白(FE)ELISA检测试剂盒标本要求:
1.标本采集后尽早进行提取,提取按相关文献进行,提取后应尽快进行实验。若不能马上进行试验,可将标本放于-20℃保存,但应避免反复冻融
2.不能检测含NaN3的样品,因NaN3抑制辣根过氧化物酶的(HRP)活性。
注意事项:
1. 试剂盒从冷藏环境中取出应在室温平衡15-30分钟后方可使用,酶标包被板开封后如未用完,板条应装入密封袋中保存。
2. 浓洗涤液可能会有结晶析出,稀释时可在水浴中加温助溶,洗涤时不影响结果。
3. 各步加样均应使用加样器,并经常校对其准确性,以避免试验误差。一次加样时间控制在5分钟内,如标本数量多,推荐使用排枪加样。
4. 请每次测定的同时做标准曲线,做复孔。如标本中待测物质含量过高(样本OD值大于标准品孔*孔的OD值),请先用样品稀释液稀释一定倍数(n倍)后再测定,计算时请zui后乘以总稀释倍数(×n×5)。
5. 封板膜只限一次性使用,以避免交叉污染。
6. 底物请避光保存。
7. 严格按照说明书的操作进行,试验结果判定必须以酶标仪读数为准.
8. 所有样品,洗涤液和各种废弃物都应按传染物处理。
9. 本试剂不同批号组分不得混用。
10. 如与英文说明书有异,以英文说明书为准。
计算:
以标准物的浓度为横坐标,OD值为纵坐标,
在坐标纸上绘出标准曲线,根据样品的OD
值由标准曲线查出相应的浓度;再乘以稀释
倍数;或用标准物的浓度与OD值计算出标
准曲线的直线回归方程式,将样品的OD值
代入方程式,计算出样品浓度,再乘以稀释
倍数,即为样品的实际浓度。
试剂盒性能:
1.样品线性回归与预期浓度相关系数R值为0.95以上。
2.批内与批见应分别小于9%和11%
保存条件及有效期:
1.试剂盒保存;2-8℃。
2.有效期:6个月 齐一生物科技有限公司主要经营进口原装Elisa试剂盒,种类齐全,价格实惠,购买我公司产品的新老客户,可享免费代测实验服务,可检测人源,小鼠,大鼠,猪,狗,猴,兔,羊,鸡,牛,鸭子,鱼,植物等各种样本。全程提供。如有什么问题请给我们或者留言,告诉我您的需要,并留下您宝贵的意见和建议。
Instrument Part 9240764 DC Output Module 8NPT, 3AMP, AP96
Instrument Part 9240668 Nut, Flangeless,1/4-28, Blue, AP
Instrument Part 9240768 Signal Conditioner, Centrifuge, AP96
Instrument Part 9018132 Splash guard, extractor, Qsym
Instrument Part 9018135 Sensor, xz, et/hs, Qsym
Instrument Part 9019901 Lead Screw - X, PyroQ96ID
Instrument Part 9019014 Heating block, EZ1xl
Instrument Part 9019312 Solenoid, door lock, EZ1xl
Instrument Part 9019023 Worktable, front bar, EZ1xl
Instrument Part 9019926 PCB, Microboard, Cxx
Instrument Part 9019927 Cover, tip ejector (UV), Cx8
Consumable 979017 MOTT 16S Primer 2 For Sequencing
950163 Final Wash Reagent (DXF) (350 ml)
Instrument Part 9021624 Kit, 8 ch pip. plunger upgrade, Cx8
Replacement Component 1031240 REPLI-g Midi-Reacti.Buffer(730μl/2),KG
Consumable 35061 Ni-NTA HisSorb Plates (5) 5 Ni-NTA-coated, transparent 96-well plates
Consumable 51504 QIAamp DNA Stool Mini Kit (50) For 50 DNA preps: 50 QIAamp Mini Spin Columns, QIAGEN Proteinase K, InhibitEX tablets, Buffers, Collection Tubes (2 ml)
Consumable 205110 Omniscript RT Kit (10) For 10 reverse-transcription reactions: 40 units Omniscript Reverse Transcriptase, 10x Buffer RT, dNTP Mix,* RNase-free water
Consumable 963141 QIAquick 96 PCR BioRobot Kit (4) For purification of 4 x 96 PCR products: 4 QIAquick 96 Plates, Reagents, Buffers, Collection Microtubes (1.2 ml) and Caps, 96-Well Microplates RB and Lids, Tape Pads
Replacement Component 1011132 RDD Buffer Set for RNeasy 96 "as per TS DE
35 ml Buffer RDD and 7 ml RNase-free water"
Instrument Part 9239151 Screw, pod fixation (to support), TWII
Instrument Part 9239362 Inlet-micro-fuse T 6.3A, 250V, EZ1
Consumable 132600 NeXtal Stock Potassium sodium tar. (50) 1.5M Potassium sodium tartrate tetrahydrate - 50mL (4 x 12,5 ml tube)
Consumable 132738 NeXtal Stock Dioxane (200) 100% v/v Dioxane - 200mL (20 x 10 ml tube)
Instrument Part 9019579 LED, power assembly, C1x
Instrument Part 9019625 PCB, level sensor rev8, Cx2
Consumable 9019088 PyroMark IdentiFire SW 1.0 (5) "Software for sequence-based species identification by paring and matching Pyrosequencing data to a local database.
Five additional software licenses for PyroMark IdentiFire SW 1.0"
Consumable 970032 PyroMark Q24 CpG MGMT (4 x 24) For quantification of methylation level in region 17-39 of exon 1 of the MGMT gene.
Consumable 979001 PyroMark Q96 Plate (100) "For sample analysis of DNA template prepared with magnetic beads for ID.
Magnetic bead technology instead of vacuum prep tool is used here"
Consumable 979203 PyroMark Control Oligo For installation check of PyroMark instrument.
Instrument Part 9019455 Upgrade kit GD100, incl. Perm/Remove, HS
Consumable 950901 Capture Plate, standard yield (5)
Instrument Part 990554 Reagent Trough (with Lid), 70 mL (10) ccc
Instrument Part 9240765 32 IN Module, D2-32ND3, AP96
Instrument Part 9240767 8 OUT Relay, F2-08TRS, AP96
Instrument Part 9240770 Assy, Capper Shuttle, AP96
Instrument Part 9017435 Sample tracking, Qsym
Instrument Part 9018149 Piercing, consumable drawer, Qsym
9018156 Tip slide, waste drawer, Qsym
Instrument Part 9018157 Holder, tip bag, waste drawer, Qsym
Instrument Part 9240950 PCB, xyz, portal, Qsym
Instrument Part 9240983 Cooling device, eluate drawer, Qsym
Instrument Part 9240984 PCB, p&c, cooling device, Qsym
929707 QX Cartridge Stand Cover (1)
Consumable 218161 miScript II RT Kit (50)
Consumable 873001 IDH 1/2 RGQ PCR kit (20)
Instrument Part 9238682 #Service manual Zent3, english
Instrument Part 9238696 LED display HD1133
Instrument Part 9238707 Temperature sensor
Instrument Part 9238785 Swing-out rotor 72 x 15 ml plete
Instrument Part 9238809 Round carrier for 4 Falcon tubes 50ml
Instrument Part 9238820 Rect. carrier for 2 tubes 50 ml
Instrument Part 9235700 Power Cable 24V, RCS1
Consumable 206145 QIAGEN Multiplex PCR Kit (1000) "contains
1 x Multiplex PCR MasterMix 2x, (1 x 25 ml)
(Mastermix contains HotStarTaq? DNA Polymerase, Multiplex PCR Buffer*, dNTP Mix)
1 x Q-Solution (10ml)
1 x RNase-free water (20 ml)
Handbook"
Instrument Part 9017186 Plunger set (6), Biorobot EZ1
9240103 BR MDX DSP, Re-Installation The Re-Installation, BR MDx DSP facilitates quick implementation of system back into lab routine by minimizing instrument set-up phase. It covers hardware and software check of instrument and provides user with operator refesher overview. Re-installation includes labor and travel costs. Re-installation performance process is documented on a GMP/GLP-pliant Field Service document.
Instrument Part 9016493 BR MDx DSP Service, PRO
Instrument Part 9239071 Valve 3-way, Kloehn
MDx 764133 PAXgene Bone Marrow RNA Kit (30) "For storage and transport of bone marrow samples and stabilization and purification of intracellular RNA
Integrated system for storage, stabilization, and purification
Immediate stabilization of intracellular RNA at collection
Rapid purification from heterogeneous bone marrow samples
Safe and easy transportation"
Consumable 337831 qBiomarker SYBR Fluor Mastermix (8)
Consumable 387116 Investigator Quantiplex HYres Kit (200)
9021147 Bottle, Reagent (with cap assy), DCU
Instrument Part 9238246 Elution Tube Rack, 1.5 ml
Instrument Part 9015076 Probe, stainless steel, 0.9mm, 138 mm
Consumable 970452 PyroMark Q24 SNP KRAS v2.0 (4 x 24) For detection of mutations in codon 12, codon 13 and codon 61.optimized for FFPE material
Consumable 972032 PyroMark Q96 CpG MGMT (96) For quantification of methylation level in region 17-39 of exon 1 of the MGMT gene.
Consumable 979006 PyroMark Binding Buffer (200 ml) For immobilization of biotinylated DNA to streptavidin coated Sepharose beads.
Consumable 979104 PyroMark Q96 HS Capillary Tip (8) "Re-usable capillary tips for dispensing nucleotides (CDTs) for use in PyroMark Q96 MD. Each package contains 8 tips
CDT = capillary dispensing tips"
Instrument Part 9019460 Heater assy incl. element & sensor, HS
Instrument Part 981400 Rotor-Disc OTV Kit (1) "Kit for optical temperature verification of Rotor-Gene systems (includes a Rotor-Disc preloaded with thermochromatic liquid crystals, fluorescent inserts, CD with calibration files)
REQUIRES ROTOR-DISC 72 ROTOR AND LOCKING RING OR ROTOR-DISC 72 STARTER KIT"
Instrument Part 9017841 Tool, rotor fixation, calibration, QIAC
Instrument Part 9022664 Channeling Block Holder, QXT
Consumable 12381 EndoFree Plasmid Mega Kit (5) 5 QIAGEN-tip 2500, Reagents, 5 QIAfilter Mega-Giga Cartridges, Endotoxin-free Buffers
Accessory 19560 Collection Microtubes (racked) Nonsterile polypropylene tubes (1.2 ml), 960 in racks of 96
Consumable 30250 Ni-NTA Agarose (500 ml) 500 ml nickel-charged resin (max. pressure: 2.8 psi)
Consumable 13343 Blood & Cell Culture DNA Midi Kit 25 QIAGEN Genomic-tip 100/G, QIAGEN Protease, Buffers
Consumable 74106 RNeasy Mini Kit (250) 250 RNeasy Mini Spin Columns, Collection Tubes (1.5 ml and 2 ml), RNase-free Reagents and Buffers
Consumable 19075 Buffer AL 216 ml for 1000 preps
Accessory 19157 QIAGEN Protease (30 AU) 4 x 7.5 Anson units per vial (lyophilized)
Accessory 19583 24-Well Blocks RB (24) 24-well blocks with 10 ml round-bottom wells, 24 per case
Consumable 28604 MinElute Gel Extraction Kit (50) 50 MinElute Spin Columns, Buffers, Collection Tubes (2 ml)
Consumable 34530 Ni-NTA HRP Conjugate Horseradish-peroxidase-conjugated Ni-NTA (lyophilized, for 500 ml working solution)
Accessory 34964 Polypropylene Columns (5-ml) 50/pack, 5 ml capacity
Instrument Part 9018095 QIAcube Transport Box
Instrument Part 9240568 Centrifuge Locator Cylinder, AP96
Instrument Part 9240649 Internal BCR, Keyence, BL-601, AP196/98
Instrument Part 9240697 Adaptor, Waste Drain, AP
Instrument Part 9240680 Spill Tray, Shaker,Modified, AP
Instrument Part 9018163 Electronic box, Qsym
Instrument Part 9018139 Tray, extractor, Qsym
Instrument Part 9018153 Waste drawer, Qsym
Instrument Part 9240968 Barcode camera, sample tracking, Qsym
Instrument Part 9240976 PCB, display, sample tracking, Qsym
Consumable 19419 QIAvac Connecting System System to connect vacuum manifold with vacuum pump: includes Tray, Waste Bottles, Tubings, Couplings, Valve, Gauge, 24 VacValves
Instrument Part 9238605 Centrifuge Motor, plete
Instrument Part 9238631 Keypad, foil
Instrument Part 9238647 Mechanical lock with optic, left