Thrombin (MW 37kDa) (Synonyms: 凝xue酶)
Thrombin (MW 37kDa) 是一种 Na+ 激huo的胰dan白酶样别构丝an酸蛋白酶。Thrombin 能够利用凝xue酶识别序列对融合蛋白进行位点特异性切割，并可用于消化 GST 标记的蛋白。
生物活性
Thrombin (MW 37kDa) is a Na+-activated, allosteric serine protease that plays opposing functional roles in blood coagulation. Thrombin recognition sequence and can be used to digest GST-tagged proteins.
体外研究(In Vitro)
p62 is cleaved from the agarose beads as well as the GST tag by addition of Thrombin (1 Unit/100 µg protein) at room temperature for 20 h[3].
Thrombin solution: Dissolve in PBS prechilled to 4°C. Swirl gently. Store solution in small aliquots at -80°C to preserve activity.
Cleavage buffer: PBS (140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4) pH 7.3.
Elution buffer: 50 mM Tris-HCl, 10 mM reduced glutathione, pH 8.0
分子量:37000(Average)
性状:Solid
CAS 号:9002-04-4
中文名称:凝xue酶
运输条件
Room temperature in continental US; may vary elsewhere
储存方式
4°C, protect from light
*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light
参考文献
[1]. Golderman V, et al. Thrombin and the Protease-Activated Receptor-1 in Organophosphate-Induced Status Epilepticus. J Mol Neurosci. 2018 Dec 4. [Content Brief]
[2]. Krenzlin H, et al. The Importance of Thrombin in Cerebral Injury and Disease. Int J Mol Sci. 2016 Jan 11;17(1). [Content Brief]
[3]. Geetha T, et al. p62 serves as a shuttling factor for TrkA interaction with the proteasome. Biochem Biophys Res Commun. 2008 Sep 12;374(1):33-7. [Content Brief]
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